Membrane:Article Title: Biomimetic nanoimmunotherapy boosts spatiotemporal PANoptosis and reshapes desmoplastic tumor microenvironment
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER (S)-Crizotinib Aladdin Cat#S288194 Tetrakis(4-carboxyphenyl)porphyrin (TCPP) TCI Cat#A5015 1-octadecene Sigma-Aldrich Cat#O806 Oleic acid Sigma-Aldrich Cat#364525 Polyethylene glycol (PEG) solution (50% (w/v)) Sigma-Aldrich Cat#P7306 Tetrahydrobiopterin (BH4, 11.212) Schircks Labs N/A Critical commercial assays Membrane and Cytosol Protein Extraction Kit Beyotime CatP0033 Reactive Oxygen Species Assay Kit Beyotime Cat#S0033 Alexa Fluor 488–conjugated avidin Thermo Fisher Cat#S11223 Cell Counting Kit-8 Beyotime Cat#C0038 Calcein/PI Cell Viability/Cytotoxicity Assay Kit Beyotime Cat#C2015 Annexin V-FITC Apoptosis Detection Kit Beyotime Cat#C1062 Apoptosis and Necrosis Detection Kit with YO-PRO-1 and PI Beyotime Cat#C1075 LDH Cytotoxicity Assay Kit Beyotime Cat#C0017 Mouse IL-1β ELISA Kit YUBIBIO Cat#U96-1494E Mouse IL-18 ELISA Kit YUBIBIO Cat#U96-3279E Deposited data Bulk RNA sequencing data This paper [NCBI]: [PRJNA1286157] Experimental models: Cell lines Murine 4T1 breast cancer cell line ATCC Cat#CRL-2539 Murine B16F10 melanoma cell line ATCC Cat#CRL-6475 Experimental models: Organisms/strains Mouse: Female BALB/c Shanghai Model Organisms Center N/A Mouse: Female C57BL/6J Shanghai Model Organisms Center N/A Mouse: Female BALB/c-nu Shanghai Model Organisms Center N/A Software and algorithms GraphPad Prism (version 9.5.1) GraphPad Software https://www.graphpad.com/ Adobe Illustrator Adobe https://www.adobe.com/products/ illustrator/free-trial-download.html ZEN Zeiss https://www.zeiss.com/microscopy/ en/products/software/zeiss-zen.html FlowJo (version 10.8.1) BD Biosciences https://www.flowjo.com/ Living Image (version 4.1) Caliper Life Sciences https://living-image.software. informer.com/4.1/ e2 Cell Reports Medicine 6, 102312, September 16, 2025 Article ll OPEN ACCESS ..
Article Title: CAR-M2 immunotherapy resolves renal fibrosis via revascularization and apoptosis of profibrotic Cxcr2 + endothelial cells.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER SDS coolaber Cat#CS9701 Triton X-100 Solution (10%, Sterile) Beyotime CatST797 Tween 20 Beyotime Cat#ST825 DiR membrane dye probe Meilunbio Cat#MB12482 Dio membrane dye probe Meilunbio Cat#MB4239-1 Western and IP cell lysates Beyotime Cat#P0013 Protease inhibitor Mixture (generic, 100×) Beyotime Cat#P1005 Protease phosphatase inhibitor mixture Beyotime Cat#P1045 RIPA lysate Beyotime Cat#P0013B Loading buffer Solarbio Cat#D8371 PVDF membrane Merck millipore Cat#IPVH0010 Matrigel Corning Cat#354234 Goat serum Beyotime Cat#C0265 DAPI dye solution Beyotime Cat#C1005 Hoechst 33342 Mengbio Cat#MBR007 4% paraformaldehyde tissue fixative Biosharp Cat#BL539A Glycine Solaribio Cat#G8200 APS Solaribio Cat#A1010-500 10%SDS Solution Solaribio Cat#S1010 1M Tris-Hcl Buffer Solaribio Cat#T1020 1.5M Tris-Hcl Buffer Solaribio Cat#T1010 OCT frozen section embedding agent SAKURA Cat#4583 Methylprenylated hyaluronic acid EFL Cat#EFL-HAMA-150K Luciferase substrate Dowobio Cat#DW3113 Microfil Flow tech Inc Cat#MV-117 DNase I Roche Cat#10104159001 Recombinant Human Flt3-Ligand PeproTech Cat#300-19 Recombinant Human GM-CSF PeproTech Cat#300-03 Recombinant Human M-CSF PeproTech Cat#300-25 Recombinant Human TGF-β1 PeproTech Cat#100-21 SB265610 MACKLIN Cat#S921626 ZINC00640089 MedChemExpress Cat#667880-11-7 GM6001 aladdin Cat#G274767 Recombinant human MMP2 protein Solarbio Cat#P02344 ANG 193109 MedChemExpress Cat#HY-U00449 LG10268 MedChemExpress Cat#HY-15340 Corn oil MedChemExpress Cat#HY-Y1888 Critical commercial assays Mouse IL-4 ELISA Kit Beyotime Cat#PI612 Mouse IL-10 ELISA Kit Beyotime Cat#PI522 Mouse IFN-γ ELISA Kit Beyotime Cat#PI508 Modified Sirius red staining kit Solarbio Cat#G1472 Modified Masson staining kit Solarbio Cat#G1346 BCA protein concentration determination kit Beyotime Cat#P0012 TSA Fluorescence Triple Staining Kit ABclonal Cat#RK05903 Cell Counting Kit-8 Beyotime Cat#C0039 Annexin V-APC / PI Apoptosis Detection Kit Elabscience Cat#E-CK-A217 10× Genomics Chromium Genetic Analyzer 10× Genomics RRID:SCR_019326 (Continued on next page) e2 Cell Reports Medicine 7, 102698, April 21, 2026 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Biotinylated Human FAP Protein, His,AvitagTM (MALS verified) ACROBiosystems Cat#FAP-H82Q6 Spherotech Streptavidin Polystyrene Particles, 0.5%w/v, 5.0–5.9 μm Spherotech Cat#SVP-50-5 Alexa Fluor 488 NHS ESTER Invitrogen Cat#A20000 Agilent Bioanalyzer Agilent RRID:SCR_018043 Biological samples Human kidney tissue slice Department of Pathology at the Eighth Medical Center of Chinese PLA General Hospital N/A Deposited data scRNA-seq data of mice This paper GEO: GSE252943 (https://www. ncbi.nlm.nih.gov/geo/query/ acc.cgi?acc=GSE252943) scRNA-seq data of human Abedini et al.53 GEO: GSE211785 (https://www. ncbi.nlm.nih.gov/geo/query/ acc.cgi?acc=GSE211785) Bulk-RNA sequencing data This paper GEO: GSE282078 (https://www. ncbi.nlm.nih.gov/geo/query/ acc.cgi?acc=GSE282078) Experimental models: Cell lines Human Umbilical Venous Endothelial Cells (HUVEC) ATCC Cat#bio-81814 Primary human renal fibroblasts icellbioscience Cat#HUM-iCell-u013 THP-1 ATCC Cat#bio-73174 Mouse bone marrow-derived macrophages (BMDMs) This paper N/A hPSC-M Jun Shen lab (State key laboratory of Experimental Hematology)61 N/A Experimental models: Organisms/strains Mouse: balb/C Shanghai Model Organisms Center Cat#SM-003 Mouse: Rosa-LSL-RSR-tdTomato-DTR Shanghai Model Organisms Center N/A Mouse: Cxcr2-DreERT2 This paper N/A Mouse: Cdh5-CreERT2 Shanghai Model Organisms Center Cat#NM-KI-200173 Mouse: Cdh5-CreERT2; Cxcr2-DreERT2; Rosa-LSL-RSR-tdTomato-DTR This paper N/A Software and algorithms ImageJ https://imagej-nihgov.gate2.inist.fr/ij/ N/A FlowJo https://www.flowjo.com 10.8.1 Prism Graphpad https://www.graphpad.com/features Version10 Cell Ranger https://10xgenomics.com/ 7.0.1 R https://www.r-project.org/ 4.1.2 Seurat https://satijalab.org/seurat 5.0.1 Harmony https://github.com/immunogenomics/ harmony 0.1.1 msigdbr https://github.com/igordot/msigdbr 7.5.1 CellChat https://github.com/sqjin/CellChat 1.6.1 SCENIC https://github.com/aertslab/SCENIC 1.3.1 devtools https://CRAN.R-project.org/ package=devtools 2.4.5 ComplexHeatmap N/A 2.9.3 (Continued on next page) Cell Reports Medicine 7, 102698, April 21, 2026 e3 Article ll OPEN ACCESS
Protein Extraction:Article Title: Biomimetic nanoimmunotherapy boosts spatiotemporal PANoptosis and reshapes desmoplastic tumor microenvironment
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER (S)-Crizotinib Aladdin Cat#S288194 Tetrakis(4-carboxyphenyl)porphyrin (TCPP) TCI Cat#A5015 1-octadecene Sigma-Aldrich Cat#O806 Oleic acid Sigma-Aldrich Cat#364525 Polyethylene glycol (PEG) solution (50% (w/v)) Sigma-Aldrich Cat#P7306 Tetrahydrobiopterin (BH4, 11.212) Schircks Labs N/A Critical commercial assays Membrane and Cytosol Protein Extraction Kit Beyotime CatP0033 Reactive Oxygen Species Assay Kit Beyotime Cat#S0033 Alexa Fluor 488–conjugated avidin Thermo Fisher Cat#S11223 Cell Counting Kit-8 Beyotime Cat#C0038 Calcein/PI Cell Viability/Cytotoxicity Assay Kit Beyotime Cat#C2015 Annexin V-FITC Apoptosis Detection Kit Beyotime Cat#C1062 Apoptosis and Necrosis Detection Kit with YO-PRO-1 and PI Beyotime Cat#C1075 LDH Cytotoxicity Assay Kit Beyotime Cat#C0017 Mouse IL-1β ELISA Kit YUBIBIO Cat#U96-1494E Mouse IL-18 ELISA Kit YUBIBIO Cat#U96-3279E Deposited data Bulk RNA sequencing data This paper [NCBI]: [PRJNA1286157] Experimental models: Cell lines Murine 4T1 breast cancer cell line ATCC Cat#CRL-2539 Murine B16F10 melanoma cell line ATCC Cat#CRL-6475 Experimental models: Organisms/strains Mouse: Female BALB/c Shanghai Model Organisms Center N/A Mouse: Female C57BL/6J Shanghai Model Organisms Center N/A Mouse: Female BALB/c-nu Shanghai Model Organisms Center N/A Software and algorithms GraphPad Prism (version 9.5.1) GraphPad Software https://www.graphpad.com/ Adobe Illustrator Adobe https://www.adobe.com/products/ illustrator/free-trial-download.html ZEN Zeiss https://www.zeiss.com/microscopy/ en/products/software/zeiss-zen.html FlowJo (version 10.8.1) BD Biosciences https://www.flowjo.com/ Living Image (version 4.1) Caliper Life Sciences https://living-image.software. informer.com/4.1/ e2 Cell Reports Medicine 6, 102312, September 16, 2025 Article ll OPEN ACCESS ..
Avidin-Biotin Assay:Article Title: Biomimetic nanoimmunotherapy boosts spatiotemporal PANoptosis and reshapes desmoplastic tumor microenvironment
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER (S)-Crizotinib Aladdin Cat#S288194 Tetrakis(4-carboxyphenyl)porphyrin (TCPP) TCI Cat#A5015 1-octadecene Sigma-Aldrich Cat#O806 Oleic acid Sigma-Aldrich Cat#364525 Polyethylene glycol (PEG) solution (50% (w/v)) Sigma-Aldrich Cat#P7306 Tetrahydrobiopterin (BH4, 11.212) Schircks Labs N/A Critical commercial assays Membrane and Cytosol Protein Extraction Kit Beyotime CatP0033 Reactive Oxygen Species Assay Kit Beyotime Cat#S0033 Alexa Fluor 488–conjugated avidin Thermo Fisher Cat#S11223 Cell Counting Kit-8 Beyotime Cat#C0038 Calcein/PI Cell Viability/Cytotoxicity Assay Kit Beyotime Cat#C2015 Annexin V-FITC Apoptosis Detection Kit Beyotime Cat#C1062 Apoptosis and Necrosis Detection Kit with YO-PRO-1 and PI Beyotime Cat#C1075 LDH Cytotoxicity Assay Kit Beyotime Cat#C0017 Mouse IL-1β ELISA Kit YUBIBIO Cat#U96-1494E Mouse IL-18 ELISA Kit YUBIBIO Cat#U96-3279E Deposited data Bulk RNA sequencing data This paper [NCBI]: [PRJNA1286157] Experimental models: Cell lines Murine 4T1 breast cancer cell line ATCC Cat#CRL-2539 Murine B16F10 melanoma cell line ATCC Cat#CRL-6475 Experimental models: Organisms/strains Mouse: Female BALB/c Shanghai Model Organisms Center N/A Mouse: Female C57BL/6J Shanghai Model Organisms Center N/A Mouse: Female BALB/c-nu Shanghai Model Organisms Center N/A Software and algorithms GraphPad Prism (version 9.5.1) GraphPad Software https://www.graphpad.com/ Adobe Illustrator Adobe https://www.adobe.com/products/ illustrator/free-trial-download.html ZEN Zeiss https://www.zeiss.com/microscopy/ en/products/software/zeiss-zen.html FlowJo (version 10.8.1) BD Biosciences https://www.flowjo.com/ Living Image (version 4.1) Caliper Life Sciences https://living-image.software. informer.com/4.1/ e2 Cell Reports Medicine 6, 102312, September 16, 2025 Article ll OPEN ACCESS ..
CCK-8 Assay:Article Title: Biomimetic nanoimmunotherapy boosts spatiotemporal PANoptosis and reshapes desmoplastic tumor microenvironment
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER (S)-Crizotinib Aladdin Cat#S288194 Tetrakis(4-carboxyphenyl)porphyrin (TCPP) TCI Cat#A5015 1-octadecene Sigma-Aldrich Cat#O806 Oleic acid Sigma-Aldrich Cat#364525 Polyethylene glycol (PEG) solution (50% (w/v)) Sigma-Aldrich Cat#P7306 Tetrahydrobiopterin (BH4, 11.212) Schircks Labs N/A Critical commercial assays Membrane and Cytosol Protein Extraction Kit Beyotime CatP0033 Reactive Oxygen Species Assay Kit Beyotime Cat#S0033 Alexa Fluor 488–conjugated avidin Thermo Fisher Cat#S11223 Cell Counting Kit-8 Beyotime Cat#C0038 Calcein/PI Cell Viability/Cytotoxicity Assay Kit Beyotime Cat#C2015 Annexin V-FITC Apoptosis Detection Kit Beyotime Cat#C1062 Apoptosis and Necrosis Detection Kit with YO-PRO-1 and PI Beyotime Cat#C1075 LDH Cytotoxicity Assay Kit Beyotime Cat#C0017 Mouse IL-1β ELISA Kit YUBIBIO Cat#U96-1494E Mouse IL-18 ELISA Kit YUBIBIO Cat#U96-3279E Deposited data Bulk RNA sequencing data This paper [NCBI]: [PRJNA1286157] Experimental models: Cell lines Murine 4T1 breast cancer cell line ATCC Cat#CRL-2539 Murine B16F10 melanoma cell line ATCC Cat#CRL-6475 Experimental models: Organisms/strains Mouse: Female BALB/c Shanghai Model Organisms Center N/A Mouse: Female C57BL/6J Shanghai Model Organisms Center N/A Mouse: Female BALB/c-nu Shanghai Model Organisms Center N/A Software and algorithms GraphPad Prism (version 9.5.1) GraphPad Software https://www.graphpad.com/ Adobe Illustrator Adobe https://www.adobe.com/products/ illustrator/free-trial-download.html ZEN Zeiss https://www.zeiss.com/microscopy/ en/products/software/zeiss-zen.html FlowJo (version 10.8.1) BD Biosciences https://www.flowjo.com/ Living Image (version 4.1) Caliper Life Sciences https://living-image.software. informer.com/4.1/ e2 Cell Reports Medicine 6, 102312, September 16, 2025 Article ll OPEN ACCESS ..
Article Title: Cancer-associated fibroblast-derived fibulin-5 promotes radioresistance in non-small-cell lung cancer.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER MEM Medium Gibco Cat#11095072 Fetal bovine serum Gibco Cat#10099-141C Exosome-Depleted Fetal Bovine Serum Gibco Cat#A2720801 Protease and Phosphatase Inhibitor Cocktail Thermo Fisher Scientific Cat#78440 BODIPY 581/591 C11 Thermo Fisher Scientific Cat#D3861 TRIzol Reagent Thermo Fisher Scientific Cat#15596026CN Lipofectamine 3000 Reagent Thermo Fisher Scientific Cat#L3000015 Protein A Magnetic Beads Thermo Fisher Scientific Cat#88845 Ferrostatin-1 MedChemExpress Cat#HY-100579 Dimethyl sulfoxide MedChemExpress Cat#HY-Y0320G Erastin MedChemExpress Cat#HY-15763 MG132 MedChemExpress Cat#HY-13259 Puromycin Solarbio Cat#P8230 DAPI Servicebio Cat#G1012 NP-40 Lysis Buffer Beyotime CatP0013F jetPRIME Versatile DNA/siRNA Transfection Reagent Polyplus Cat#101000046 STAT3 Inhibitor VIII, 5,15-DPP Sigma-Aldrich Cat#573109 Recombinant Human Fibulin 5 Protein R&D Systems Cat#9006-FB-050 Critical commercial assays Annexin V-FITC Cell Apoptosis Detection Kit BD Biosciences Cat#556547 Reactive Oxygen Species Assay Kit Beyotime Cat#S0033M Cell Cycle and Apoptosis Analysis Kit Beyotime Cat#C1052 Cell Counting Kit-8 Beyotime Cat#C0038 BCA Protein Assay Kit Beyotime Cat#P0012 Lipid Peroxidation MDA Assay Kit Beyotime Cat#S0131M Total Exosome Isolation Reagent (from cell culture media) Thermo Fisher Scientific Cat#4478359 Dihydroethidium Thermo Fisher Scientific Cat#D11347 PKH67 Green Fluorescent Cell Linker Mini Kit Sigma-Aldrich Cat#MINI67 Dual-Luciferase® Reporter Assay System Promega, Madison, Wisconsin. .. Cat#E1910 AceQ Universal SYBR qPCR Master Mix Vazyme Cat#Q511-02 HiScript III RT SuperMix for qPCR (+gDNA wiper) Vazyme Cat#R323-01 Lipid Peroxidation MDA Assay Kit Beyotime Cat#S0130M ECL Western Blotting Substrate Thermo Fisher Scientific Cat#32106 Deposited data Digital spatial transcriptomics profiling This paper GSA: HRA010680 RNA-seq This paper GSA: HRA010129 MicroRNA seq This paper GSA: CRA022306 Single-cell RNA-seq This paper GSA: CRA022568 Experimental models: cell lines A549 ATCC Cat#CCL-185; RRID: CVCL_0023 MRC5 ATCC Cat#CCL-171; RRID: CVCL_0440 LLC ATCC Cat#CRL-1642; RRID: CVCL_4358 NIH-3T3 ATCC Cat#CRL-1658; RRID: CVCL_0594 (Continued on next page) Cell Reports 44, 116018, August 26, 2025 19
Article Title: CAR-M2 immunotherapy resolves renal fibrosis via revascularization and apoptosis of profibrotic Cxcr2 + endothelial cells.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER SDS coolaber Cat#CS9701 Triton X-100 Solution (10%, Sterile) Beyotime CatST797 Tween 20 Beyotime Cat#ST825 DiR membrane dye probe Meilunbio Cat#MB12482 Dio membrane dye probe Meilunbio Cat#MB4239-1 Western and IP cell lysates Beyotime Cat#P0013 Protease inhibitor Mixture (generic, 100×) Beyotime Cat#P1005 Protease phosphatase inhibitor mixture Beyotime Cat#P1045 RIPA lysate Beyotime Cat#P0013B Loading buffer Solarbio Cat#D8371 PVDF membrane Merck millipore Cat#IPVH0010 Matrigel Corning Cat#354234 Goat serum Beyotime Cat#C0265 DAPI dye solution Beyotime Cat#C1005 Hoechst 33342 Mengbio Cat#MBR007 4% paraformaldehyde tissue fixative Biosharp Cat#BL539A Glycine Solaribio Cat#G8200 APS Solaribio Cat#A1010-500 10%SDS Solution Solaribio Cat#S1010 1M Tris-Hcl Buffer Solaribio Cat#T1020 1.5M Tris-Hcl Buffer Solaribio Cat#T1010 OCT frozen section embedding agent SAKURA Cat#4583 Methylprenylated hyaluronic acid EFL Cat#EFL-HAMA-150K Luciferase substrate Dowobio Cat#DW3113 Microfil Flow tech Inc Cat#MV-117 DNase I Roche Cat#10104159001 Recombinant Human Flt3-Ligand PeproTech Cat#300-19 Recombinant Human GM-CSF PeproTech Cat#300-03 Recombinant Human M-CSF PeproTech Cat#300-25 Recombinant Human TGF-β1 PeproTech Cat#100-21 SB265610 MACKLIN Cat#S921626 ZINC00640089 MedChemExpress Cat#667880-11-7 GM6001 aladdin Cat#G274767 Recombinant human MMP2 protein Solarbio Cat#P02344 ANG 193109 MedChemExpress Cat#HY-U00449 LG10268 MedChemExpress Cat#HY-15340 Corn oil MedChemExpress Cat#HY-Y1888 Critical commercial assays Mouse IL-4 ELISA Kit Beyotime Cat#PI612 Mouse IL-10 ELISA Kit Beyotime Cat#PI522 Mouse IFN-γ ELISA Kit Beyotime Cat#PI508 Modified Sirius red staining kit Solarbio Cat#G1472 Modified Masson staining kit Solarbio Cat#G1346 BCA protein concentration determination kit Beyotime Cat#P0012 TSA Fluorescence Triple Staining Kit ABclonal Cat#RK05903 Cell Counting Kit-8 Beyotime Cat#C0039 Annexin V-APC / PI Apoptosis Detection Kit Elabscience Cat#E-CK-A217 10× Genomics Chromium Genetic Analyzer 10× Genomics RRID:SCR_019326 (Continued on next page) e2 Cell Reports Medicine 7, 102698, April 21, 2026 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Biotinylated Human FAP Protein, His,AvitagTM (MALS verified) ACROBiosystems Cat#FAP-H82Q6 Spherotech Streptavidin Polystyrene Particles, 0.5%w/v, 5.0–5.9 μm Spherotech Cat#SVP-50-5 Alexa Fluor 488 NHS ESTER Invitrogen Cat#A20000 Agilent Bioanalyzer Agilent RRID:SCR_018043 Biological samples Human kidney tissue slice Department of Pathology at the Eighth Medical Center of Chinese PLA General Hospital N/A Deposited data scRNA-seq data of mice This paper GEO: GSE252943 (https://www. ncbi.nlm.nih.gov/geo/query/ acc.cgi?acc=GSE252943) scRNA-seq data of human Abedini et al.53 GEO: GSE211785 (https://www. ncbi.nlm.nih.gov/geo/query/ acc.cgi?acc=GSE211785) Bulk-RNA sequencing data This paper GEO: GSE282078 (https://www. ncbi.nlm.nih.gov/geo/query/ acc.cgi?acc=GSE282078) Experimental models: Cell lines Human Umbilical Venous Endothelial Cells (HUVEC) ATCC Cat#bio-81814 Primary human renal fibroblasts icellbioscience Cat#HUM-iCell-u013 THP-1 ATCC Cat#bio-73174 Mouse bone marrow-derived macrophages (BMDMs) This paper N/A hPSC-M Jun Shen lab (State key laboratory of Experimental Hematology)61 N/A Experimental models: Organisms/strains Mouse: balb/C Shanghai Model Organisms Center Cat#SM-003 Mouse: Rosa-LSL-RSR-tdTomato-DTR Shanghai Model Organisms Center N/A Mouse: Cxcr2-DreERT2 This paper N/A Mouse: Cdh5-CreERT2 Shanghai Model Organisms Center Cat#NM-KI-200173 Mouse: Cdh5-CreERT2; Cxcr2-DreERT2; Rosa-LSL-RSR-tdTomato-DTR This paper N/A Software and algorithms ImageJ https://imagej-nihgov.gate2.inist.fr/ij/ N/A FlowJo https://www.flowjo.com 10.8.1 Prism Graphpad https://www.graphpad.com/features Version10 Cell Ranger https://10xgenomics.com/ 7.0.1 R https://www.r-project.org/ 4.1.2 Seurat https://satijalab.org/seurat 5.0.1 Harmony https://github.com/immunogenomics/ harmony 0.1.1 msigdbr https://github.com/igordot/msigdbr 7.5.1 CellChat https://github.com/sqjin/CellChat 1.6.1 SCENIC https://github.com/aertslab/SCENIC 1.3.1 devtools https://CRAN.R-project.org/ package=devtools 2.4.5 ComplexHeatmap N/A 2.9.3 (Continued on next page) Cell Reports Medicine 7, 102698, April 21, 2026 e3 Article ll OPEN ACCESS
Article Title: METTL3 regulates α-KG-dependent mitophagy and apoptosis in nucleus pulposus cells through the MALAT1/miR-23c/IDH1 axis.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-ACAN Affinity Cat#DF7561; RRID: AB_2841055 Rabbit anti-COLL II Proteintech Cat#28459-1-AP; RRID: AB_2881147 Rabbit anti-MMP13 Proteintech Cat#18165-1-AP; RRID: AB_2144858 Rabbit anti-ADAMTS5 Affinity Cat#DF13268; RRID: AB_2846287 Rabbit anti-GAPDH Proteintech Cat#60004-1-Ig; RRID: AB_2107436 Rabbit anti-IL-6 Abcam Cat#ab233706; RRID: AB_2889391 Rabbit anti-TNF-α Abcam Cat#ab183218; RRID: AB_2889388 Rabbit anti-Cleaved PARP-1 ThermoFisher Cat#44-698G; RRID: AB_2533726 Rabbit anti-Cleaved Caspase-3 Cell Signaling Technology Cat#9661; RRID: AB_2341188 Rabbit anti-BAX Proteintech Cat#50599-2; RRID: AB_2061561 Rabbit anti-BCL2 Affinity Cat#AF6139; RRID: AB_2835021 Rabbit anti-CAT Abclonal Cat#A11777; RRID: AB_2758747 Rabbit anti-SOD1 Abcam Cat#ab13498; RRID: AB_300402 Rabbit anti-p62 Proteintech Cat#18420-1-AP; RRID: AB_10694431 Rabbit anti-LC3B-II Cell Signaling Technology Cat#2775S; RRID: AB_915950 Rabbit anti-PINK1 Abcam Cat#ab216144; RRID: AB_2927726 Rabbit anti-Parkin Abcam Cat#ab315376; RRID: AB_3668762 Rabbit anti-p-Parkin Thermo Fisher Cat#PA5-114616; RRID: AB_2899252 Rabbit anti-Ubiquitin-Ser65 Boston Biochem Cat#A-110; RRID: AB_2636932 Rabbit anti-IDH1 Abcam Cat#ab256557; RRID: AB_3099653 Rabbit anti-IDH2 Abcam Cat#ab131263; RRID: AB_11156098 Rabbit anti-METTL3 Cell Signaling Technology Cat#86132; RRID: AB_2800072 Biological samples Human nucleus pulposus tissue This paper N/A HEK 293T cells ATCC Cat# CRL-11268 Chemicals, peptides, and recombinant proteins Type II collagenase Invitrogen Cat#17101015 DMEM/F12 medium Gibco Cat#11320033 Fetal bovine serum Gibco Cat#10099141C Penicillin/streptomycin Gibco Cat#15140122 TRIzol reagent Invitrogen Cat15596018CN RIPA Lysis Buffer Beyotime Cat#P0013B Phenylmethanesulfonyl fluoride Beyotime Cat#ST505 DAPI Solarbio Cat#C0065 Actinomycin D MedChem Cat#HY-17559 QuickBlock TM Blocking Buffer Beyotime Cat#P0220 Lipofectamine TM 2000 Invitrogen Cat#11668019 Critical commercial assays α-KG Assay Kit GraceBiotechnology Cat#G0861W Cell Counting Kit-8 Beyotime Cat#C0037 Senescence β-Galactosidase Staining Kit Beyotime Cat#C0602 EdU Cell Proliferation Kit with Alexa Fluor 594 Beyotime Cat#C0078S BCA Protein Assay Kit Beyotime Cat#P0011 (Continued on next page) 20 Cell Reports 45, 116793, January 27, 2026 .. Small pieces of NP tissue that were intraoperatively collected were subjected to 8 h of digestion with 0.2% concentration of type II collagenase (Invitrogen, USA) at 37 ◦ C, followed by 5 min of centrifugation at 1200 rpm.
LDH Cytotoxicity Assay:Article Title: Biomimetic nanoimmunotherapy boosts spatiotemporal PANoptosis and reshapes desmoplastic tumor microenvironment
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER (S)-Crizotinib Aladdin Cat#S288194 Tetrakis(4-carboxyphenyl)porphyrin (TCPP) TCI Cat#A5015 1-octadecene Sigma-Aldrich Cat#O806 Oleic acid Sigma-Aldrich Cat#364525 Polyethylene glycol (PEG) solution (50% (w/v)) Sigma-Aldrich Cat#P7306 Tetrahydrobiopterin (BH4, 11.212) Schircks Labs N/A Critical commercial assays Membrane and Cytosol Protein Extraction Kit Beyotime CatP0033 Reactive Oxygen Species Assay Kit Beyotime Cat#S0033 Alexa Fluor 488–conjugated avidin Thermo Fisher Cat#S11223 Cell Counting Kit-8 Beyotime Cat#C0038 Calcein/PI Cell Viability/Cytotoxicity Assay Kit Beyotime Cat#C2015 Annexin V-FITC Apoptosis Detection Kit Beyotime Cat#C1062 Apoptosis and Necrosis Detection Kit with YO-PRO-1 and PI Beyotime Cat#C1075 LDH Cytotoxicity Assay Kit Beyotime Cat#C0017 Mouse IL-1β ELISA Kit YUBIBIO Cat#U96-1494E Mouse IL-18 ELISA Kit YUBIBIO Cat#U96-3279E Deposited data Bulk RNA sequencing data This paper [NCBI]: [PRJNA1286157] Experimental models: Cell lines Murine 4T1 breast cancer cell line ATCC Cat#CRL-2539 Murine B16F10 melanoma cell line ATCC Cat#CRL-6475 Experimental models: Organisms/strains Mouse: Female BALB/c Shanghai Model Organisms Center N/A Mouse: Female C57BL/6J Shanghai Model Organisms Center N/A Mouse: Female BALB/c-nu Shanghai Model Organisms Center N/A Software and algorithms GraphPad Prism (version 9.5.1) GraphPad Software https://www.graphpad.com/ Adobe Illustrator Adobe https://www.adobe.com/products/ illustrator/free-trial-download.html ZEN Zeiss https://www.zeiss.com/microscopy/ en/products/software/zeiss-zen.html FlowJo (version 10.8.1) BD Biosciences https://www.flowjo.com/ Living Image (version 4.1) Caliper Life Sciences https://living-image.software. informer.com/4.1/ e2 Cell Reports Medicine 6, 102312, September 16, 2025 Article ll OPEN ACCESS ..
Enzyme-linked Immunosorbent Assay:Article Title: Biomimetic nanoimmunotherapy boosts spatiotemporal PANoptosis and reshapes desmoplastic tumor microenvironment
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER (S)-Crizotinib Aladdin Cat#S288194 Tetrakis(4-carboxyphenyl)porphyrin (TCPP) TCI Cat#A5015 1-octadecene Sigma-Aldrich Cat#O806 Oleic acid Sigma-Aldrich Cat#364525 Polyethylene glycol (PEG) solution (50% (w/v)) Sigma-Aldrich Cat#P7306 Tetrahydrobiopterin (BH4, 11.212) Schircks Labs N/A Critical commercial assays Membrane and Cytosol Protein Extraction Kit Beyotime CatP0033 Reactive Oxygen Species Assay Kit Beyotime Cat#S0033 Alexa Fluor 488–conjugated avidin Thermo Fisher Cat#S11223 Cell Counting Kit-8 Beyotime Cat#C0038 Calcein/PI Cell Viability/Cytotoxicity Assay Kit Beyotime Cat#C2015 Annexin V-FITC Apoptosis Detection Kit Beyotime Cat#C1062 Apoptosis and Necrosis Detection Kit with YO-PRO-1 and PI Beyotime Cat#C1075 LDH Cytotoxicity Assay Kit Beyotime Cat#C0017 Mouse IL-1β ELISA Kit YUBIBIO Cat#U96-1494E Mouse IL-18 ELISA Kit YUBIBIO Cat#U96-3279E Deposited data Bulk RNA sequencing data This paper [NCBI]: [PRJNA1286157] Experimental models: Cell lines Murine 4T1 breast cancer cell line ATCC Cat#CRL-2539 Murine B16F10 melanoma cell line ATCC Cat#CRL-6475 Experimental models: Organisms/strains Mouse: Female BALB/c Shanghai Model Organisms Center N/A Mouse: Female C57BL/6J Shanghai Model Organisms Center N/A Mouse: Female BALB/c-nu Shanghai Model Organisms Center N/A Software and algorithms GraphPad Prism (version 9.5.1) GraphPad Software https://www.graphpad.com/ Adobe Illustrator Adobe https://www.adobe.com/products/ illustrator/free-trial-download.html ZEN Zeiss https://www.zeiss.com/microscopy/ en/products/software/zeiss-zen.html FlowJo (version 10.8.1) BD Biosciences https://www.flowjo.com/ Living Image (version 4.1) Caliper Life Sciences https://living-image.software. informer.com/4.1/ e2 Cell Reports Medicine 6, 102312, September 16, 2025 Article ll OPEN ACCESS ..
Article Title: CAR-M2 immunotherapy resolves renal fibrosis via revascularization and apoptosis of profibrotic Cxcr2 + endothelial cells.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER SDS coolaber Cat#CS9701 Triton X-100 Solution (10%, Sterile) Beyotime CatST797 Tween 20 Beyotime Cat#ST825 DiR membrane dye probe Meilunbio Cat#MB12482 Dio membrane dye probe Meilunbio Cat#MB4239-1 Western and IP cell lysates Beyotime Cat#P0013 Protease inhibitor Mixture (generic, 100×) Beyotime Cat#P1005 Protease phosphatase inhibitor mixture Beyotime Cat#P1045 RIPA lysate Beyotime Cat#P0013B Loading buffer Solarbio Cat#D8371 PVDF membrane Merck millipore Cat#IPVH0010 Matrigel Corning Cat#354234 Goat serum Beyotime Cat#C0265 DAPI dye solution Beyotime Cat#C1005 Hoechst 33342 Mengbio Cat#MBR007 4% paraformaldehyde tissue fixative Biosharp Cat#BL539A Glycine Solaribio Cat#G8200 APS Solaribio Cat#A1010-500 10%SDS Solution Solaribio Cat#S1010 1M Tris-Hcl Buffer Solaribio Cat#T1020 1.5M Tris-Hcl Buffer Solaribio Cat#T1010 OCT frozen section embedding agent SAKURA Cat#4583 Methylprenylated hyaluronic acid EFL Cat#EFL-HAMA-150K Luciferase substrate Dowobio Cat#DW3113 Microfil Flow tech Inc Cat#MV-117 DNase I Roche Cat#10104159001 Recombinant Human Flt3-Ligand PeproTech Cat#300-19 Recombinant Human GM-CSF PeproTech Cat#300-03 Recombinant Human M-CSF PeproTech Cat#300-25 Recombinant Human TGF-β1 PeproTech Cat#100-21 SB265610 MACKLIN Cat#S921626 ZINC00640089 MedChemExpress Cat#667880-11-7 GM6001 aladdin Cat#G274767 Recombinant human MMP2 protein Solarbio Cat#P02344 ANG 193109 MedChemExpress Cat#HY-U00449 LG10268 MedChemExpress Cat#HY-15340 Corn oil MedChemExpress Cat#HY-Y1888 Critical commercial assays Mouse IL-4 ELISA Kit Beyotime Cat#PI612 Mouse IL-10 ELISA Kit Beyotime Cat#PI522 Mouse IFN-γ ELISA Kit Beyotime Cat#PI508 Modified Sirius red staining kit Solarbio Cat#G1472 Modified Masson staining kit Solarbio Cat#G1346 BCA protein concentration determination kit Beyotime Cat#P0012 TSA Fluorescence Triple Staining Kit ABclonal Cat#RK05903 Cell Counting Kit-8 Beyotime Cat#C0039 Annexin V-APC / PI Apoptosis Detection Kit Elabscience Cat#E-CK-A217 10× Genomics Chromium Genetic Analyzer 10× Genomics RRID:SCR_019326 (Continued on next page) e2 Cell Reports Medicine 7, 102698, April 21, 2026 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Biotinylated Human FAP Protein, His,AvitagTM (MALS verified) ACROBiosystems Cat#FAP-H82Q6 Spherotech Streptavidin Polystyrene Particles, 0.5%w/v, 5.0–5.9 μm Spherotech Cat#SVP-50-5 Alexa Fluor 488 NHS ESTER Invitrogen Cat#A20000 Agilent Bioanalyzer Agilent RRID:SCR_018043 Biological samples Human kidney tissue slice Department of Pathology at the Eighth Medical Center of Chinese PLA General Hospital N/A Deposited data scRNA-seq data of mice This paper GEO: GSE252943 (https://www. ncbi.nlm.nih.gov/geo/query/ acc.cgi?acc=GSE252943) scRNA-seq data of human Abedini et al.53 GEO: GSE211785 (https://www. ncbi.nlm.nih.gov/geo/query/ acc.cgi?acc=GSE211785) Bulk-RNA sequencing data This paper GEO: GSE282078 (https://www. ncbi.nlm.nih.gov/geo/query/ acc.cgi?acc=GSE282078) Experimental models: Cell lines Human Umbilical Venous Endothelial Cells (HUVEC) ATCC Cat#bio-81814 Primary human renal fibroblasts icellbioscience Cat#HUM-iCell-u013 THP-1 ATCC Cat#bio-73174 Mouse bone marrow-derived macrophages (BMDMs) This paper N/A hPSC-M Jun Shen lab (State key laboratory of Experimental Hematology)61 N/A Experimental models: Organisms/strains Mouse: balb/C Shanghai Model Organisms Center Cat#SM-003 Mouse: Rosa-LSL-RSR-tdTomato-DTR Shanghai Model Organisms Center N/A Mouse: Cxcr2-DreERT2 This paper N/A Mouse: Cdh5-CreERT2 Shanghai Model Organisms Center Cat#NM-KI-200173 Mouse: Cdh5-CreERT2; Cxcr2-DreERT2; Rosa-LSL-RSR-tdTomato-DTR This paper N/A Software and algorithms ImageJ https://imagej-nihgov.gate2.inist.fr/ij/ N/A FlowJo https://www.flowjo.com 10.8.1 Prism Graphpad https://www.graphpad.com/features Version10 Cell Ranger https://10xgenomics.com/ 7.0.1 R https://www.r-project.org/ 4.1.2 Seurat https://satijalab.org/seurat 5.0.1 Harmony https://github.com/immunogenomics/ harmony 0.1.1 msigdbr https://github.com/igordot/msigdbr 7.5.1 CellChat https://github.com/sqjin/CellChat 1.6.1 SCENIC https://github.com/aertslab/SCENIC 1.3.1 devtools https://CRAN.R-project.org/ package=devtools 2.4.5 ComplexHeatmap N/A 2.9.3 (Continued on next page) Cell Reports Medicine 7, 102698, April 21, 2026 e3 Article ll OPEN ACCESS
RNA Sequencing:Article Title: Biomimetic nanoimmunotherapy boosts spatiotemporal PANoptosis and reshapes desmoplastic tumor microenvironment
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER (S)-Crizotinib Aladdin Cat#S288194 Tetrakis(4-carboxyphenyl)porphyrin (TCPP) TCI Cat#A5015 1-octadecene Sigma-Aldrich Cat#O806 Oleic acid Sigma-Aldrich Cat#364525 Polyethylene glycol (PEG) solution (50% (w/v)) Sigma-Aldrich Cat#P7306 Tetrahydrobiopterin (BH4, 11.212) Schircks Labs N/A Critical commercial assays Membrane and Cytosol Protein Extraction Kit Beyotime CatP0033 Reactive Oxygen Species Assay Kit Beyotime Cat#S0033 Alexa Fluor 488–conjugated avidin Thermo Fisher Cat#S11223 Cell Counting Kit-8 Beyotime Cat#C0038 Calcein/PI Cell Viability/Cytotoxicity Assay Kit Beyotime Cat#C2015 Annexin V-FITC Apoptosis Detection Kit Beyotime Cat#C1062 Apoptosis and Necrosis Detection Kit with YO-PRO-1 and PI Beyotime Cat#C1075 LDH Cytotoxicity Assay Kit Beyotime Cat#C0017 Mouse IL-1β ELISA Kit YUBIBIO Cat#U96-1494E Mouse IL-18 ELISA Kit YUBIBIO Cat#U96-3279E Deposited data Bulk RNA sequencing data This paper [NCBI]: [PRJNA1286157] Experimental models: Cell lines Murine 4T1 breast cancer cell line ATCC Cat#CRL-2539 Murine B16F10 melanoma cell line ATCC Cat#CRL-6475 Experimental models: Organisms/strains Mouse: Female BALB/c Shanghai Model Organisms Center N/A Mouse: Female C57BL/6J Shanghai Model Organisms Center N/A Mouse: Female BALB/c-nu Shanghai Model Organisms Center N/A Software and algorithms GraphPad Prism (version 9.5.1) GraphPad Software https://www.graphpad.com/ Adobe Illustrator Adobe https://www.adobe.com/products/ illustrator/free-trial-download.html ZEN Zeiss https://www.zeiss.com/microscopy/ en/products/software/zeiss-zen.html FlowJo (version 10.8.1) BD Biosciences https://www.flowjo.com/ Living Image (version 4.1) Caliper Life Sciences https://living-image.software. informer.com/4.1/ e2 Cell Reports Medicine 6, 102312, September 16, 2025 Article ll OPEN ACCESS ..
Software:Article Title: Biomimetic nanoimmunotherapy boosts spatiotemporal PANoptosis and reshapes desmoplastic tumor microenvironment
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER (S)-Crizotinib Aladdin Cat#S288194 Tetrakis(4-carboxyphenyl)porphyrin (TCPP) TCI Cat#A5015 1-octadecene Sigma-Aldrich Cat#O806 Oleic acid Sigma-Aldrich Cat#364525 Polyethylene glycol (PEG) solution (50% (w/v)) Sigma-Aldrich Cat#P7306 Tetrahydrobiopterin (BH4, 11.212) Schircks Labs N/A Critical commercial assays Membrane and Cytosol Protein Extraction Kit Beyotime CatP0033 Reactive Oxygen Species Assay Kit Beyotime Cat#S0033 Alexa Fluor 488–conjugated avidin Thermo Fisher Cat#S11223 Cell Counting Kit-8 Beyotime Cat#C0038 Calcein/PI Cell Viability/Cytotoxicity Assay Kit Beyotime Cat#C2015 Annexin V-FITC Apoptosis Detection Kit Beyotime Cat#C1062 Apoptosis and Necrosis Detection Kit with YO-PRO-1 and PI Beyotime Cat#C1075 LDH Cytotoxicity Assay Kit Beyotime Cat#C0017 Mouse IL-1β ELISA Kit YUBIBIO Cat#U96-1494E Mouse IL-18 ELISA Kit YUBIBIO Cat#U96-3279E Deposited data Bulk RNA sequencing data This paper [NCBI]: [PRJNA1286157] Experimental models: Cell lines Murine 4T1 breast cancer cell line ATCC Cat#CRL-2539 Murine B16F10 melanoma cell line ATCC Cat#CRL-6475 Experimental models: Organisms/strains Mouse: Female BALB/c Shanghai Model Organisms Center N/A Mouse: Female C57BL/6J Shanghai Model Organisms Center N/A Mouse: Female BALB/c-nu Shanghai Model Organisms Center N/A Software and algorithms GraphPad Prism (version 9.5.1) GraphPad Software https://www.graphpad.com/ Adobe Illustrator Adobe https://www.adobe.com/products/ illustrator/free-trial-download.html ZEN Zeiss https://www.zeiss.com/microscopy/ en/products/software/zeiss-zen.html FlowJo (version 10.8.1) BD Biosciences https://www.flowjo.com/ Living Image (version 4.1) Caliper Life Sciences https://living-image.software. informer.com/4.1/ e2 Cell Reports Medicine 6, 102312, September 16, 2025 Article ll OPEN ACCESS ..
Magnetic Beads:Article Title: Cancer-associated fibroblast-derived fibulin-5 promotes radioresistance in non-small-cell lung cancer.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER MEM Medium Gibco Cat#11095072 Fetal bovine serum Gibco Cat#10099-141C Exosome-Depleted Fetal Bovine Serum Gibco Cat#A2720801 Protease and Phosphatase Inhibitor Cocktail Thermo Fisher Scientific Cat#78440 BODIPY 581/591 C11 Thermo Fisher Scientific Cat#D3861 TRIzol Reagent Thermo Fisher Scientific Cat#15596026CN Lipofectamine 3000 Reagent Thermo Fisher Scientific Cat#L3000015 Protein A Magnetic Beads Thermo Fisher Scientific Cat#88845 Ferrostatin-1 MedChemExpress Cat#HY-100579 Dimethyl sulfoxide MedChemExpress Cat#HY-Y0320G Erastin MedChemExpress Cat#HY-15763 MG132 MedChemExpress Cat#HY-13259 Puromycin Solarbio Cat#P8230 DAPI Servicebio Cat#G1012 NP-40 Lysis Buffer Beyotime CatP0013F jetPRIME Versatile DNA/siRNA Transfection Reagent Polyplus Cat#101000046 STAT3 Inhibitor VIII, 5,15-DPP Sigma-Aldrich Cat#573109 Recombinant Human Fibulin 5 Protein R&D Systems Cat#9006-FB-050 Critical commercial assays Annexin V-FITC Cell Apoptosis Detection Kit BD Biosciences Cat#556547 Reactive Oxygen Species Assay Kit Beyotime Cat#S0033M Cell Cycle and Apoptosis Analysis Kit Beyotime Cat#C1052 Cell Counting Kit-8 Beyotime Cat#C0038 BCA Protein Assay Kit Beyotime Cat#P0012 Lipid Peroxidation MDA Assay Kit Beyotime Cat#S0131M Total Exosome Isolation Reagent (from cell culture media) Thermo Fisher Scientific Cat#4478359 Dihydroethidium Thermo Fisher Scientific Cat#D11347 PKH67 Green Fluorescent Cell Linker Mini Kit Sigma-Aldrich Cat#MINI67 Dual-Luciferase® Reporter Assay System Promega, Madison, Wisconsin. .. Cat#E1910 AceQ Universal SYBR qPCR Master Mix Vazyme Cat#Q511-02 HiScript III RT SuperMix for qPCR (+gDNA wiper) Vazyme Cat#R323-01 Lipid Peroxidation MDA Assay Kit Beyotime Cat#S0130M ECL Western Blotting Substrate Thermo Fisher Scientific Cat#32106 Deposited data Digital spatial transcriptomics profiling This paper GSA: HRA010680 RNA-seq This paper GSA: HRA010129 MicroRNA seq This paper GSA: CRA022306 Single-cell RNA-seq This paper GSA: CRA022568 Experimental models: cell lines A549 ATCC Cat#CCL-185; RRID: CVCL_0023 MRC5 ATCC Cat#CCL-171; RRID: CVCL_0440 LLC ATCC Cat#CRL-1642; RRID: CVCL_4358 NIH-3T3 ATCC Cat#CRL-1658; RRID: CVCL_0594 (Continued on next page) Cell Reports 44, 116018, August 26, 2025 19
Lysis:Article Title: Cancer-associated fibroblast-derived fibulin-5 promotes radioresistance in non-small-cell lung cancer.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER MEM Medium Gibco Cat#11095072 Fetal bovine serum Gibco Cat#10099-141C Exosome-Depleted Fetal Bovine Serum Gibco Cat#A2720801 Protease and Phosphatase Inhibitor Cocktail Thermo Fisher Scientific Cat#78440 BODIPY 581/591 C11 Thermo Fisher Scientific Cat#D3861 TRIzol Reagent Thermo Fisher Scientific Cat#15596026CN Lipofectamine 3000 Reagent Thermo Fisher Scientific Cat#L3000015 Protein A Magnetic Beads Thermo Fisher Scientific Cat#88845 Ferrostatin-1 MedChemExpress Cat#HY-100579 Dimethyl sulfoxide MedChemExpress Cat#HY-Y0320G Erastin MedChemExpress Cat#HY-15763 MG132 MedChemExpress Cat#HY-13259 Puromycin Solarbio Cat#P8230 DAPI Servicebio Cat#G1012 NP-40 Lysis Buffer Beyotime CatP0013F jetPRIME Versatile DNA/siRNA Transfection Reagent Polyplus Cat#101000046 STAT3 Inhibitor VIII, 5,15-DPP Sigma-Aldrich Cat#573109 Recombinant Human Fibulin 5 Protein R&D Systems Cat#9006-FB-050 Critical commercial assays Annexin V-FITC Cell Apoptosis Detection Kit BD Biosciences Cat#556547 Reactive Oxygen Species Assay Kit Beyotime Cat#S0033M Cell Cycle and Apoptosis Analysis Kit Beyotime Cat#C1052 Cell Counting Kit-8 Beyotime Cat#C0038 BCA Protein Assay Kit Beyotime Cat#P0012 Lipid Peroxidation MDA Assay Kit Beyotime Cat#S0131M Total Exosome Isolation Reagent (from cell culture media) Thermo Fisher Scientific Cat#4478359 Dihydroethidium Thermo Fisher Scientific Cat#D11347 PKH67 Green Fluorescent Cell Linker Mini Kit Sigma-Aldrich Cat#MINI67 Dual-Luciferase® Reporter Assay System Promega, Madison, Wisconsin. .. Cat#E1910 AceQ Universal SYBR qPCR Master Mix Vazyme Cat#Q511-02 HiScript III RT SuperMix for qPCR (+gDNA wiper) Vazyme Cat#R323-01 Lipid Peroxidation MDA Assay Kit Beyotime Cat#S0130M ECL Western Blotting Substrate Thermo Fisher Scientific Cat#32106 Deposited data Digital spatial transcriptomics profiling This paper GSA: HRA010680 RNA-seq This paper GSA: HRA010129 MicroRNA seq This paper GSA: CRA022306 Single-cell RNA-seq This paper GSA: CRA022568 Experimental models: cell lines A549 ATCC Cat#CCL-185; RRID: CVCL_0023 MRC5 ATCC Cat#CCL-171; RRID: CVCL_0440 LLC ATCC Cat#CRL-1642; RRID: CVCL_4358 NIH-3T3 ATCC Cat#CRL-1658; RRID: CVCL_0594 (Continued on next page) Cell Reports 44, 116018, August 26, 2025 19
Article Title: METTL3 regulates α-KG-dependent mitophagy and apoptosis in nucleus pulposus cells through the MALAT1/miR-23c/IDH1 axis.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-ACAN Affinity Cat#DF7561; RRID: AB_2841055 Rabbit anti-COLL II Proteintech Cat#28459-1-AP; RRID: AB_2881147 Rabbit anti-MMP13 Proteintech Cat#18165-1-AP; RRID: AB_2144858 Rabbit anti-ADAMTS5 Affinity Cat#DF13268; RRID: AB_2846287 Rabbit anti-GAPDH Proteintech Cat#60004-1-Ig; RRID: AB_2107436 Rabbit anti-IL-6 Abcam Cat#ab233706; RRID: AB_2889391 Rabbit anti-TNF-α Abcam Cat#ab183218; RRID: AB_2889388 Rabbit anti-Cleaved PARP-1 ThermoFisher Cat#44-698G; RRID: AB_2533726 Rabbit anti-Cleaved Caspase-3 Cell Signaling Technology Cat#9661; RRID: AB_2341188 Rabbit anti-BAX Proteintech Cat#50599-2; RRID: AB_2061561 Rabbit anti-BCL2 Affinity Cat#AF6139; RRID: AB_2835021 Rabbit anti-CAT Abclonal Cat#A11777; RRID: AB_2758747 Rabbit anti-SOD1 Abcam Cat#ab13498; RRID: AB_300402 Rabbit anti-p62 Proteintech Cat#18420-1-AP; RRID: AB_10694431 Rabbit anti-LC3B-II Cell Signaling Technology Cat#2775S; RRID: AB_915950 Rabbit anti-PINK1 Abcam Cat#ab216144; RRID: AB_2927726 Rabbit anti-Parkin Abcam Cat#ab315376; RRID: AB_3668762 Rabbit anti-p-Parkin Thermo Fisher Cat#PA5-114616; RRID: AB_2899252 Rabbit anti-Ubiquitin-Ser65 Boston Biochem Cat#A-110; RRID: AB_2636932 Rabbit anti-IDH1 Abcam Cat#ab256557; RRID: AB_3099653 Rabbit anti-IDH2 Abcam Cat#ab131263; RRID: AB_11156098 Rabbit anti-METTL3 Cell Signaling Technology Cat#86132; RRID: AB_2800072 Biological samples Human nucleus pulposus tissue This paper N/A HEK 293T cells ATCC Cat# CRL-11268 Chemicals, peptides, and recombinant proteins Type II collagenase Invitrogen Cat#17101015 DMEM/F12 medium Gibco Cat#11320033 Fetal bovine serum Gibco Cat#10099141C Penicillin/streptomycin Gibco Cat#15140122 TRIzol reagent Invitrogen Cat15596018CN RIPA Lysis Buffer Beyotime Cat#P0013B Phenylmethanesulfonyl fluoride Beyotime Cat#ST505 DAPI Solarbio Cat#C0065 Actinomycin D MedChem Cat#HY-17559 QuickBlock TM Blocking Buffer Beyotime Cat#P0220 Lipofectamine TM 2000 Invitrogen Cat#11668019 Critical commercial assays α-KG Assay Kit GraceBiotechnology Cat#G0861W Cell Counting Kit-8 Beyotime Cat#C0037 Senescence β-Galactosidase Staining Kit Beyotime Cat#C0602 EdU Cell Proliferation Kit with Alexa Fluor 594 Beyotime Cat#C0078S BCA Protein Assay Kit Beyotime Cat#P0011 (Continued on next page) 20 Cell Reports 45, 116793, January 27, 2026 .. Small pieces of NP tissue that were intraoperatively collected were subjected to 8 h of digestion with 0.2% concentration of type II collagenase (Invitrogen, USA) at 37 ◦ C, followed by 5 min of centrifugation at 1200 rpm.
Transfection:Article Title: Cancer-associated fibroblast-derived fibulin-5 promotes radioresistance in non-small-cell lung cancer.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER MEM Medium Gibco Cat#11095072 Fetal bovine serum Gibco Cat#10099-141C Exosome-Depleted Fetal Bovine Serum Gibco Cat#A2720801 Protease and Phosphatase Inhibitor Cocktail Thermo Fisher Scientific Cat#78440 BODIPY 581/591 C11 Thermo Fisher Scientific Cat#D3861 TRIzol Reagent Thermo Fisher Scientific Cat#15596026CN Lipofectamine 3000 Reagent Thermo Fisher Scientific Cat#L3000015 Protein A Magnetic Beads Thermo Fisher Scientific Cat#88845 Ferrostatin-1 MedChemExpress Cat#HY-100579 Dimethyl sulfoxide MedChemExpress Cat#HY-Y0320G Erastin MedChemExpress Cat#HY-15763 MG132 MedChemExpress Cat#HY-13259 Puromycin Solarbio Cat#P8230 DAPI Servicebio Cat#G1012 NP-40 Lysis Buffer Beyotime CatP0013F jetPRIME Versatile DNA/siRNA Transfection Reagent Polyplus Cat#101000046 STAT3 Inhibitor VIII, 5,15-DPP Sigma-Aldrich Cat#573109 Recombinant Human Fibulin 5 Protein R&D Systems Cat#9006-FB-050 Critical commercial assays Annexin V-FITC Cell Apoptosis Detection Kit BD Biosciences Cat#556547 Reactive Oxygen Species Assay Kit Beyotime Cat#S0033M Cell Cycle and Apoptosis Analysis Kit Beyotime Cat#C1052 Cell Counting Kit-8 Beyotime Cat#C0038 BCA Protein Assay Kit Beyotime Cat#P0012 Lipid Peroxidation MDA Assay Kit Beyotime Cat#S0131M Total Exosome Isolation Reagent (from cell culture media) Thermo Fisher Scientific Cat#4478359 Dihydroethidium Thermo Fisher Scientific Cat#D11347 PKH67 Green Fluorescent Cell Linker Mini Kit Sigma-Aldrich Cat#MINI67 Dual-Luciferase® Reporter Assay System Promega, Madison, Wisconsin. .. Cat#E1910 AceQ Universal SYBR qPCR Master Mix Vazyme Cat#Q511-02 HiScript III RT SuperMix for qPCR (+gDNA wiper) Vazyme Cat#R323-01 Lipid Peroxidation MDA Assay Kit Beyotime Cat#S0130M ECL Western Blotting Substrate Thermo Fisher Scientific Cat#32106 Deposited data Digital spatial transcriptomics profiling This paper GSA: HRA010680 RNA-seq This paper GSA: HRA010129 MicroRNA seq This paper GSA: CRA022306 Single-cell RNA-seq This paper GSA: CRA022568 Experimental models: cell lines A549 ATCC Cat#CCL-185; RRID: CVCL_0023 MRC5 ATCC Cat#CCL-171; RRID: CVCL_0440 LLC ATCC Cat#CRL-1642; RRID: CVCL_4358 NIH-3T3 ATCC Cat#CRL-1658; RRID: CVCL_0594 (Continued on next page) Cell Reports 44, 116018, August 26, 2025 19
Recombinant:Article Title: Cancer-associated fibroblast-derived fibulin-5 promotes radioresistance in non-small-cell lung cancer.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER MEM Medium Gibco Cat#11095072 Fetal bovine serum Gibco Cat#10099-141C Exosome-Depleted Fetal Bovine Serum Gibco Cat#A2720801 Protease and Phosphatase Inhibitor Cocktail Thermo Fisher Scientific Cat#78440 BODIPY 581/591 C11 Thermo Fisher Scientific Cat#D3861 TRIzol Reagent Thermo Fisher Scientific Cat#15596026CN Lipofectamine 3000 Reagent Thermo Fisher Scientific Cat#L3000015 Protein A Magnetic Beads Thermo Fisher Scientific Cat#88845 Ferrostatin-1 MedChemExpress Cat#HY-100579 Dimethyl sulfoxide MedChemExpress Cat#HY-Y0320G Erastin MedChemExpress Cat#HY-15763 MG132 MedChemExpress Cat#HY-13259 Puromycin Solarbio Cat#P8230 DAPI Servicebio Cat#G1012 NP-40 Lysis Buffer Beyotime CatP0013F jetPRIME Versatile DNA/siRNA Transfection Reagent Polyplus Cat#101000046 STAT3 Inhibitor VIII, 5,15-DPP Sigma-Aldrich Cat#573109 Recombinant Human Fibulin 5 Protein R&D Systems Cat#9006-FB-050 Critical commercial assays Annexin V-FITC Cell Apoptosis Detection Kit BD Biosciences Cat#556547 Reactive Oxygen Species Assay Kit Beyotime Cat#S0033M Cell Cycle and Apoptosis Analysis Kit Beyotime Cat#C1052 Cell Counting Kit-8 Beyotime Cat#C0038 BCA Protein Assay Kit Beyotime Cat#P0012 Lipid Peroxidation MDA Assay Kit Beyotime Cat#S0131M Total Exosome Isolation Reagent (from cell culture media) Thermo Fisher Scientific Cat#4478359 Dihydroethidium Thermo Fisher Scientific Cat#D11347 PKH67 Green Fluorescent Cell Linker Mini Kit Sigma-Aldrich Cat#MINI67 Dual-Luciferase® Reporter Assay System Promega, Madison, Wisconsin. .. Cat#E1910 AceQ Universal SYBR qPCR Master Mix Vazyme Cat#Q511-02 HiScript III RT SuperMix for qPCR (+gDNA wiper) Vazyme Cat#R323-01 Lipid Peroxidation MDA Assay Kit Beyotime Cat#S0130M ECL Western Blotting Substrate Thermo Fisher Scientific Cat#32106 Deposited data Digital spatial transcriptomics profiling This paper GSA: HRA010680 RNA-seq This paper GSA: HRA010129 MicroRNA seq This paper GSA: CRA022306 Single-cell RNA-seq This paper GSA: CRA022568 Experimental models: cell lines A549 ATCC Cat#CCL-185; RRID: CVCL_0023 MRC5 ATCC Cat#CCL-171; RRID: CVCL_0440 LLC ATCC Cat#CRL-1642; RRID: CVCL_4358 NIH-3T3 ATCC Cat#CRL-1658; RRID: CVCL_0594 (Continued on next page) Cell Reports 44, 116018, August 26, 2025 19
Article Title: CAR-M2 immunotherapy resolves renal fibrosis via revascularization and apoptosis of profibrotic Cxcr2 + endothelial cells.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER SDS coolaber Cat#CS9701 Triton X-100 Solution (10%, Sterile) Beyotime CatST797 Tween 20 Beyotime Cat#ST825 DiR membrane dye probe Meilunbio Cat#MB12482 Dio membrane dye probe Meilunbio Cat#MB4239-1 Western and IP cell lysates Beyotime Cat#P0013 Protease inhibitor Mixture (generic, 100×) Beyotime Cat#P1005 Protease phosphatase inhibitor mixture Beyotime Cat#P1045 RIPA lysate Beyotime Cat#P0013B Loading buffer Solarbio Cat#D8371 PVDF membrane Merck millipore Cat#IPVH0010 Matrigel Corning Cat#354234 Goat serum Beyotime Cat#C0265 DAPI dye solution Beyotime Cat#C1005 Hoechst 33342 Mengbio Cat#MBR007 4% paraformaldehyde tissue fixative Biosharp Cat#BL539A Glycine Solaribio Cat#G8200 APS Solaribio Cat#A1010-500 10%SDS Solution Solaribio Cat#S1010 1M Tris-Hcl Buffer Solaribio Cat#T1020 1.5M Tris-Hcl Buffer Solaribio Cat#T1010 OCT frozen section embedding agent SAKURA Cat#4583 Methylprenylated hyaluronic acid EFL Cat#EFL-HAMA-150K Luciferase substrate Dowobio Cat#DW3113 Microfil Flow tech Inc Cat#MV-117 DNase I Roche Cat#10104159001 Recombinant Human Flt3-Ligand PeproTech Cat#300-19 Recombinant Human GM-CSF PeproTech Cat#300-03 Recombinant Human M-CSF PeproTech Cat#300-25 Recombinant Human TGF-β1 PeproTech Cat#100-21 SB265610 MACKLIN Cat#S921626 ZINC00640089 MedChemExpress Cat#667880-11-7 GM6001 aladdin Cat#G274767 Recombinant human MMP2 protein Solarbio Cat#P02344 ANG 193109 MedChemExpress Cat#HY-U00449 LG10268 MedChemExpress Cat#HY-15340 Corn oil MedChemExpress Cat#HY-Y1888 Critical commercial assays Mouse IL-4 ELISA Kit Beyotime Cat#PI612 Mouse IL-10 ELISA Kit Beyotime Cat#PI522 Mouse IFN-γ ELISA Kit Beyotime Cat#PI508 Modified Sirius red staining kit Solarbio Cat#G1472 Modified Masson staining kit Solarbio Cat#G1346 BCA protein concentration determination kit Beyotime Cat#P0012 TSA Fluorescence Triple Staining Kit ABclonal Cat#RK05903 Cell Counting Kit-8 Beyotime Cat#C0039 Annexin V-APC / PI Apoptosis Detection Kit Elabscience Cat#E-CK-A217 10× Genomics Chromium Genetic Analyzer 10× Genomics RRID:SCR_019326 (Continued on next page) e2 Cell Reports Medicine 7, 102698, April 21, 2026 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Biotinylated Human FAP Protein, His,AvitagTM (MALS verified) ACROBiosystems Cat#FAP-H82Q6 Spherotech Streptavidin Polystyrene Particles, 0.5%w/v, 5.0–5.9 μm Spherotech Cat#SVP-50-5 Alexa Fluor 488 NHS ESTER Invitrogen Cat#A20000 Agilent Bioanalyzer Agilent RRID:SCR_018043 Biological samples Human kidney tissue slice Department of Pathology at the Eighth Medical Center of Chinese PLA General Hospital N/A Deposited data scRNA-seq data of mice This paper GEO: GSE252943 (https://www. ncbi.nlm.nih.gov/geo/query/ acc.cgi?acc=GSE252943) scRNA-seq data of human Abedini et al.53 GEO: GSE211785 (https://www. ncbi.nlm.nih.gov/geo/query/ acc.cgi?acc=GSE211785) Bulk-RNA sequencing data This paper GEO: GSE282078 (https://www. ncbi.nlm.nih.gov/geo/query/ acc.cgi?acc=GSE282078) Experimental models: Cell lines Human Umbilical Venous Endothelial Cells (HUVEC) ATCC Cat#bio-81814 Primary human renal fibroblasts icellbioscience Cat#HUM-iCell-u013 THP-1 ATCC Cat#bio-73174 Mouse bone marrow-derived macrophages (BMDMs) This paper N/A hPSC-M Jun Shen lab (State key laboratory of Experimental Hematology)61 N/A Experimental models: Organisms/strains Mouse: balb/C Shanghai Model Organisms Center Cat#SM-003 Mouse: Rosa-LSL-RSR-tdTomato-DTR Shanghai Model Organisms Center N/A Mouse: Cxcr2-DreERT2 This paper N/A Mouse: Cdh5-CreERT2 Shanghai Model Organisms Center Cat#NM-KI-200173 Mouse: Cdh5-CreERT2; Cxcr2-DreERT2; Rosa-LSL-RSR-tdTomato-DTR This paper N/A Software and algorithms ImageJ https://imagej-nihgov.gate2.inist.fr/ij/ N/A FlowJo https://www.flowjo.com 10.8.1 Prism Graphpad https://www.graphpad.com/features Version10 Cell Ranger https://10xgenomics.com/ 7.0.1 R https://www.r-project.org/ 4.1.2 Seurat https://satijalab.org/seurat 5.0.1 Harmony https://github.com/immunogenomics/ harmony 0.1.1 msigdbr https://github.com/igordot/msigdbr 7.5.1 CellChat https://github.com/sqjin/CellChat 1.6.1 SCENIC https://github.com/aertslab/SCENIC 1.3.1 devtools https://CRAN.R-project.org/ package=devtools 2.4.5 ComplexHeatmap N/A 2.9.3 (Continued on next page) Cell Reports Medicine 7, 102698, April 21, 2026 e3 Article ll OPEN ACCESS
Article Title: METTL3 regulates α-KG-dependent mitophagy and apoptosis in nucleus pulposus cells through the MALAT1/miR-23c/IDH1 axis.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-ACAN Affinity Cat#DF7561; RRID: AB_2841055 Rabbit anti-COLL II Proteintech Cat#28459-1-AP; RRID: AB_2881147 Rabbit anti-MMP13 Proteintech Cat#18165-1-AP; RRID: AB_2144858 Rabbit anti-ADAMTS5 Affinity Cat#DF13268; RRID: AB_2846287 Rabbit anti-GAPDH Proteintech Cat#60004-1-Ig; RRID: AB_2107436 Rabbit anti-IL-6 Abcam Cat#ab233706; RRID: AB_2889391 Rabbit anti-TNF-α Abcam Cat#ab183218; RRID: AB_2889388 Rabbit anti-Cleaved PARP-1 ThermoFisher Cat#44-698G; RRID: AB_2533726 Rabbit anti-Cleaved Caspase-3 Cell Signaling Technology Cat#9661; RRID: AB_2341188 Rabbit anti-BAX Proteintech Cat#50599-2; RRID: AB_2061561 Rabbit anti-BCL2 Affinity Cat#AF6139; RRID: AB_2835021 Rabbit anti-CAT Abclonal Cat#A11777; RRID: AB_2758747 Rabbit anti-SOD1 Abcam Cat#ab13498; RRID: AB_300402 Rabbit anti-p62 Proteintech Cat#18420-1-AP; RRID: AB_10694431 Rabbit anti-LC3B-II Cell Signaling Technology Cat#2775S; RRID: AB_915950 Rabbit anti-PINK1 Abcam Cat#ab216144; RRID: AB_2927726 Rabbit anti-Parkin Abcam Cat#ab315376; RRID: AB_3668762 Rabbit anti-p-Parkin Thermo Fisher Cat#PA5-114616; RRID: AB_2899252 Rabbit anti-Ubiquitin-Ser65 Boston Biochem Cat#A-110; RRID: AB_2636932 Rabbit anti-IDH1 Abcam Cat#ab256557; RRID: AB_3099653 Rabbit anti-IDH2 Abcam Cat#ab131263; RRID: AB_11156098 Rabbit anti-METTL3 Cell Signaling Technology Cat#86132; RRID: AB_2800072 Biological samples Human nucleus pulposus tissue This paper N/A HEK 293T cells ATCC Cat# CRL-11268 Chemicals, peptides, and recombinant proteins Type II collagenase Invitrogen Cat#17101015 DMEM/F12 medium Gibco Cat#11320033 Fetal bovine serum Gibco Cat#10099141C Penicillin/streptomycin Gibco Cat#15140122 TRIzol reagent Invitrogen Cat15596018CN RIPA Lysis Buffer Beyotime Cat#P0013B Phenylmethanesulfonyl fluoride Beyotime Cat#ST505 DAPI Solarbio Cat#C0065 Actinomycin D MedChem Cat#HY-17559 QuickBlock TM Blocking Buffer Beyotime Cat#P0220 Lipofectamine TM 2000 Invitrogen Cat#11668019 Critical commercial assays α-KG Assay Kit GraceBiotechnology Cat#G0861W Cell Counting Kit-8 Beyotime Cat#C0037 Senescence β-Galactosidase Staining Kit Beyotime Cat#C0602 EdU Cell Proliferation Kit with Alexa Fluor 594 Beyotime Cat#C0078S BCA Protein Assay Kit Beyotime Cat#P0011 (Continued on next page) 20 Cell Reports 45, 116793, January 27, 2026 .. Small pieces of NP tissue that were intraoperatively collected were subjected to 8 h of digestion with 0.2% concentration of type II collagenase (Invitrogen, USA) at 37 ◦ C, followed by 5 min of centrifugation at 1200 rpm.
Bicinchoninic Acid Protein Assay:Article Title: Cancer-associated fibroblast-derived fibulin-5 promotes radioresistance in non-small-cell lung cancer.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER MEM Medium Gibco Cat#11095072 Fetal bovine serum Gibco Cat#10099-141C Exosome-Depleted Fetal Bovine Serum Gibco Cat#A2720801 Protease and Phosphatase Inhibitor Cocktail Thermo Fisher Scientific Cat#78440 BODIPY 581/591 C11 Thermo Fisher Scientific Cat#D3861 TRIzol Reagent Thermo Fisher Scientific Cat#15596026CN Lipofectamine 3000 Reagent Thermo Fisher Scientific Cat#L3000015 Protein A Magnetic Beads Thermo Fisher Scientific Cat#88845 Ferrostatin-1 MedChemExpress Cat#HY-100579 Dimethyl sulfoxide MedChemExpress Cat#HY-Y0320G Erastin MedChemExpress Cat#HY-15763 MG132 MedChemExpress Cat#HY-13259 Puromycin Solarbio Cat#P8230 DAPI Servicebio Cat#G1012 NP-40 Lysis Buffer Beyotime CatP0013F jetPRIME Versatile DNA/siRNA Transfection Reagent Polyplus Cat#101000046 STAT3 Inhibitor VIII, 5,15-DPP Sigma-Aldrich Cat#573109 Recombinant Human Fibulin 5 Protein R&D Systems Cat#9006-FB-050 Critical commercial assays Annexin V-FITC Cell Apoptosis Detection Kit BD Biosciences Cat#556547 Reactive Oxygen Species Assay Kit Beyotime Cat#S0033M Cell Cycle and Apoptosis Analysis Kit Beyotime Cat#C1052 Cell Counting Kit-8 Beyotime Cat#C0038 BCA Protein Assay Kit Beyotime Cat#P0012 Lipid Peroxidation MDA Assay Kit Beyotime Cat#S0131M Total Exosome Isolation Reagent (from cell culture media) Thermo Fisher Scientific Cat#4478359 Dihydroethidium Thermo Fisher Scientific Cat#D11347 PKH67 Green Fluorescent Cell Linker Mini Kit Sigma-Aldrich Cat#MINI67 Dual-Luciferase® Reporter Assay System Promega, Madison, Wisconsin. .. Cat#E1910 AceQ Universal SYBR qPCR Master Mix Vazyme Cat#Q511-02 HiScript III RT SuperMix for qPCR (+gDNA wiper) Vazyme Cat#R323-01 Lipid Peroxidation MDA Assay Kit Beyotime Cat#S0130M ECL Western Blotting Substrate Thermo Fisher Scientific Cat#32106 Deposited data Digital spatial transcriptomics profiling This paper GSA: HRA010680 RNA-seq This paper GSA: HRA010129 MicroRNA seq This paper GSA: CRA022306 Single-cell RNA-seq This paper GSA: CRA022568 Experimental models: cell lines A549 ATCC Cat#CCL-185; RRID: CVCL_0023 MRC5 ATCC Cat#CCL-171; RRID: CVCL_0440 LLC ATCC Cat#CRL-1642; RRID: CVCL_4358 NIH-3T3 ATCC Cat#CRL-1658; RRID: CVCL_0594 (Continued on next page) Cell Reports 44, 116018, August 26, 2025 19
Article Title: METTL3 regulates α-KG-dependent mitophagy and apoptosis in nucleus pulposus cells through the MALAT1/miR-23c/IDH1 axis.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-ACAN Affinity Cat#DF7561; RRID: AB_2841055 Rabbit anti-COLL II Proteintech Cat#28459-1-AP; RRID: AB_2881147 Rabbit anti-MMP13 Proteintech Cat#18165-1-AP; RRID: AB_2144858 Rabbit anti-ADAMTS5 Affinity Cat#DF13268; RRID: AB_2846287 Rabbit anti-GAPDH Proteintech Cat#60004-1-Ig; RRID: AB_2107436 Rabbit anti-IL-6 Abcam Cat#ab233706; RRID: AB_2889391 Rabbit anti-TNF-α Abcam Cat#ab183218; RRID: AB_2889388 Rabbit anti-Cleaved PARP-1 ThermoFisher Cat#44-698G; RRID: AB_2533726 Rabbit anti-Cleaved Caspase-3 Cell Signaling Technology Cat#9661; RRID: AB_2341188 Rabbit anti-BAX Proteintech Cat#50599-2; RRID: AB_2061561 Rabbit anti-BCL2 Affinity Cat#AF6139; RRID: AB_2835021 Rabbit anti-CAT Abclonal Cat#A11777; RRID: AB_2758747 Rabbit anti-SOD1 Abcam Cat#ab13498; RRID: AB_300402 Rabbit anti-p62 Proteintech Cat#18420-1-AP; RRID: AB_10694431 Rabbit anti-LC3B-II Cell Signaling Technology Cat#2775S; RRID: AB_915950 Rabbit anti-PINK1 Abcam Cat#ab216144; RRID: AB_2927726 Rabbit anti-Parkin Abcam Cat#ab315376; RRID: AB_3668762 Rabbit anti-p-Parkin Thermo Fisher Cat#PA5-114616; RRID: AB_2899252 Rabbit anti-Ubiquitin-Ser65 Boston Biochem Cat#A-110; RRID: AB_2636932 Rabbit anti-IDH1 Abcam Cat#ab256557; RRID: AB_3099653 Rabbit anti-IDH2 Abcam Cat#ab131263; RRID: AB_11156098 Rabbit anti-METTL3 Cell Signaling Technology Cat#86132; RRID: AB_2800072 Biological samples Human nucleus pulposus tissue This paper N/A HEK 293T cells ATCC Cat# CRL-11268 Chemicals, peptides, and recombinant proteins Type II collagenase Invitrogen Cat#17101015 DMEM/F12 medium Gibco Cat#11320033 Fetal bovine serum Gibco Cat#10099141C Penicillin/streptomycin Gibco Cat#15140122 TRIzol reagent Invitrogen Cat15596018CN RIPA Lysis Buffer Beyotime Cat#P0013B Phenylmethanesulfonyl fluoride Beyotime Cat#ST505 DAPI Solarbio Cat#C0065 Actinomycin D MedChem Cat#HY-17559 QuickBlock TM Blocking Buffer Beyotime Cat#P0220 Lipofectamine TM 2000 Invitrogen Cat#11668019 Critical commercial assays α-KG Assay Kit GraceBiotechnology Cat#G0861W Cell Counting Kit-8 Beyotime Cat#C0037 Senescence β-Galactosidase Staining Kit Beyotime Cat#C0602 EdU Cell Proliferation Kit with Alexa Fluor 594 Beyotime Cat#C0078S BCA Protein Assay Kit Beyotime Cat#P0011 (Continued on next page) 20 Cell Reports 45, 116793, January 27, 2026 .. Small pieces of NP tissue that were intraoperatively collected were subjected to 8 h of digestion with 0.2% concentration of type II collagenase (Invitrogen, USA) at 37 ◦ C, followed by 5 min of centrifugation at 1200 rpm.
Multiple Displacement Amplification:Article Title: Cancer-associated fibroblast-derived fibulin-5 promotes radioresistance in non-small-cell lung cancer.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER MEM Medium Gibco Cat#11095072 Fetal bovine serum Gibco Cat#10099-141C Exosome-Depleted Fetal Bovine Serum Gibco Cat#A2720801 Protease and Phosphatase Inhibitor Cocktail Thermo Fisher Scientific Cat#78440 BODIPY 581/591 C11 Thermo Fisher Scientific Cat#D3861 TRIzol Reagent Thermo Fisher Scientific Cat#15596026CN Lipofectamine 3000 Reagent Thermo Fisher Scientific Cat#L3000015 Protein A Magnetic Beads Thermo Fisher Scientific Cat#88845 Ferrostatin-1 MedChemExpress Cat#HY-100579 Dimethyl sulfoxide MedChemExpress Cat#HY-Y0320G Erastin MedChemExpress Cat#HY-15763 MG132 MedChemExpress Cat#HY-13259 Puromycin Solarbio Cat#P8230 DAPI Servicebio Cat#G1012 NP-40 Lysis Buffer Beyotime CatP0013F jetPRIME Versatile DNA/siRNA Transfection Reagent Polyplus Cat#101000046 STAT3 Inhibitor VIII, 5,15-DPP Sigma-Aldrich Cat#573109 Recombinant Human Fibulin 5 Protein R&D Systems Cat#9006-FB-050 Critical commercial assays Annexin V-FITC Cell Apoptosis Detection Kit BD Biosciences Cat#556547 Reactive Oxygen Species Assay Kit Beyotime Cat#S0033M Cell Cycle and Apoptosis Analysis Kit Beyotime Cat#C1052 Cell Counting Kit-8 Beyotime Cat#C0038 BCA Protein Assay Kit Beyotime Cat#P0012 Lipid Peroxidation MDA Assay Kit Beyotime Cat#S0131M Total Exosome Isolation Reagent (from cell culture media) Thermo Fisher Scientific Cat#4478359 Dihydroethidium Thermo Fisher Scientific Cat#D11347 PKH67 Green Fluorescent Cell Linker Mini Kit Sigma-Aldrich Cat#MINI67 Dual-Luciferase® Reporter Assay System Promega, Madison, Wisconsin. .. Cat#E1910 AceQ Universal SYBR qPCR Master Mix Vazyme Cat#Q511-02 HiScript III RT SuperMix for qPCR (+gDNA wiper) Vazyme Cat#R323-01 Lipid Peroxidation MDA Assay Kit Beyotime Cat#S0130M ECL Western Blotting Substrate Thermo Fisher Scientific Cat#32106 Deposited data Digital spatial transcriptomics profiling This paper GSA: HRA010680 RNA-seq This paper GSA: HRA010129 MicroRNA seq This paper GSA: CRA022306 Single-cell RNA-seq This paper GSA: CRA022568 Experimental models: cell lines A549 ATCC Cat#CCL-185; RRID: CVCL_0023 MRC5 ATCC Cat#CCL-171; RRID: CVCL_0440 LLC ATCC Cat#CRL-1642; RRID: CVCL_4358 NIH-3T3 ATCC Cat#CRL-1658; RRID: CVCL_0594 (Continued on next page) Cell Reports 44, 116018, August 26, 2025 19
Isolation:Article Title: Cancer-associated fibroblast-derived fibulin-5 promotes radioresistance in non-small-cell lung cancer.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER MEM Medium Gibco Cat#11095072 Fetal bovine serum Gibco Cat#10099-141C Exosome-Depleted Fetal Bovine Serum Gibco Cat#A2720801 Protease and Phosphatase Inhibitor Cocktail Thermo Fisher Scientific Cat#78440 BODIPY 581/591 C11 Thermo Fisher Scientific Cat#D3861 TRIzol Reagent Thermo Fisher Scientific Cat#15596026CN Lipofectamine 3000 Reagent Thermo Fisher Scientific Cat#L3000015 Protein A Magnetic Beads Thermo Fisher Scientific Cat#88845 Ferrostatin-1 MedChemExpress Cat#HY-100579 Dimethyl sulfoxide MedChemExpress Cat#HY-Y0320G Erastin MedChemExpress Cat#HY-15763 MG132 MedChemExpress Cat#HY-13259 Puromycin Solarbio Cat#P8230 DAPI Servicebio Cat#G1012 NP-40 Lysis Buffer Beyotime CatP0013F jetPRIME Versatile DNA/siRNA Transfection Reagent Polyplus Cat#101000046 STAT3 Inhibitor VIII, 5,15-DPP Sigma-Aldrich Cat#573109 Recombinant Human Fibulin 5 Protein R&D Systems Cat#9006-FB-050 Critical commercial assays Annexin V-FITC Cell Apoptosis Detection Kit BD Biosciences Cat#556547 Reactive Oxygen Species Assay Kit Beyotime Cat#S0033M Cell Cycle and Apoptosis Analysis Kit Beyotime Cat#C1052 Cell Counting Kit-8 Beyotime Cat#C0038 BCA Protein Assay Kit Beyotime Cat#P0012 Lipid Peroxidation MDA Assay Kit Beyotime Cat#S0131M Total Exosome Isolation Reagent (from cell culture media) Thermo Fisher Scientific Cat#4478359 Dihydroethidium Thermo Fisher Scientific Cat#D11347 PKH67 Green Fluorescent Cell Linker Mini Kit Sigma-Aldrich Cat#MINI67 Dual-Luciferase® Reporter Assay System Promega, Madison, Wisconsin. .. Cat#E1910 AceQ Universal SYBR qPCR Master Mix Vazyme Cat#Q511-02 HiScript III RT SuperMix for qPCR (+gDNA wiper) Vazyme Cat#R323-01 Lipid Peroxidation MDA Assay Kit Beyotime Cat#S0130M ECL Western Blotting Substrate Thermo Fisher Scientific Cat#32106 Deposited data Digital spatial transcriptomics profiling This paper GSA: HRA010680 RNA-seq This paper GSA: HRA010129 MicroRNA seq This paper GSA: CRA022306 Single-cell RNA-seq This paper GSA: CRA022568 Experimental models: cell lines A549 ATCC Cat#CCL-185; RRID: CVCL_0023 MRC5 ATCC Cat#CCL-171; RRID: CVCL_0440 LLC ATCC Cat#CRL-1642; RRID: CVCL_4358 NIH-3T3 ATCC Cat#CRL-1658; RRID: CVCL_0594 (Continued on next page) Cell Reports 44, 116018, August 26, 2025 19
Cell Culture:Article Title: Cancer-associated fibroblast-derived fibulin-5 promotes radioresistance in non-small-cell lung cancer.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER MEM Medium Gibco Cat#11095072 Fetal bovine serum Gibco Cat#10099-141C Exosome-Depleted Fetal Bovine Serum Gibco Cat#A2720801 Protease and Phosphatase Inhibitor Cocktail Thermo Fisher Scientific Cat#78440 BODIPY 581/591 C11 Thermo Fisher Scientific Cat#D3861 TRIzol Reagent Thermo Fisher Scientific Cat#15596026CN Lipofectamine 3000 Reagent Thermo Fisher Scientific Cat#L3000015 Protein A Magnetic Beads Thermo Fisher Scientific Cat#88845 Ferrostatin-1 MedChemExpress Cat#HY-100579 Dimethyl sulfoxide MedChemExpress Cat#HY-Y0320G Erastin MedChemExpress Cat#HY-15763 MG132 MedChemExpress Cat#HY-13259 Puromycin Solarbio Cat#P8230 DAPI Servicebio Cat#G1012 NP-40 Lysis Buffer Beyotime CatP0013F jetPRIME Versatile DNA/siRNA Transfection Reagent Polyplus Cat#101000046 STAT3 Inhibitor VIII, 5,15-DPP Sigma-Aldrich Cat#573109 Recombinant Human Fibulin 5 Protein R&D Systems Cat#9006-FB-050 Critical commercial assays Annexin V-FITC Cell Apoptosis Detection Kit BD Biosciences Cat#556547 Reactive Oxygen Species Assay Kit Beyotime Cat#S0033M Cell Cycle and Apoptosis Analysis Kit Beyotime Cat#C1052 Cell Counting Kit-8 Beyotime Cat#C0038 BCA Protein Assay Kit Beyotime Cat#P0012 Lipid Peroxidation MDA Assay Kit Beyotime Cat#S0131M Total Exosome Isolation Reagent (from cell culture media) Thermo Fisher Scientific Cat#4478359 Dihydroethidium Thermo Fisher Scientific Cat#D11347 PKH67 Green Fluorescent Cell Linker Mini Kit Sigma-Aldrich Cat#MINI67 Dual-Luciferase® Reporter Assay System Promega, Madison, Wisconsin. .. Cat#E1910 AceQ Universal SYBR qPCR Master Mix Vazyme Cat#Q511-02 HiScript III RT SuperMix for qPCR (+gDNA wiper) Vazyme Cat#R323-01 Lipid Peroxidation MDA Assay Kit Beyotime Cat#S0130M ECL Western Blotting Substrate Thermo Fisher Scientific Cat#32106 Deposited data Digital spatial transcriptomics profiling This paper GSA: HRA010680 RNA-seq This paper GSA: HRA010129 MicroRNA seq This paper GSA: CRA022306 Single-cell RNA-seq This paper GSA: CRA022568 Experimental models: cell lines A549 ATCC Cat#CCL-185; RRID: CVCL_0023 MRC5 ATCC Cat#CCL-171; RRID: CVCL_0440 LLC ATCC Cat#CRL-1642; RRID: CVCL_4358 NIH-3T3 ATCC Cat#CRL-1658; RRID: CVCL_0594 (Continued on next page) Cell Reports 44, 116018, August 26, 2025 19
Reporter Assay:Article Title: Cancer-associated fibroblast-derived fibulin-5 promotes radioresistance in non-small-cell lung cancer.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER MEM Medium Gibco Cat#11095072 Fetal bovine serum Gibco Cat#10099-141C Exosome-Depleted Fetal Bovine Serum Gibco Cat#A2720801 Protease and Phosphatase Inhibitor Cocktail Thermo Fisher Scientific Cat#78440 BODIPY 581/591 C11 Thermo Fisher Scientific Cat#D3861 TRIzol Reagent Thermo Fisher Scientific Cat#15596026CN Lipofectamine 3000 Reagent Thermo Fisher Scientific Cat#L3000015 Protein A Magnetic Beads Thermo Fisher Scientific Cat#88845 Ferrostatin-1 MedChemExpress Cat#HY-100579 Dimethyl sulfoxide MedChemExpress Cat#HY-Y0320G Erastin MedChemExpress Cat#HY-15763 MG132 MedChemExpress Cat#HY-13259 Puromycin Solarbio Cat#P8230 DAPI Servicebio Cat#G1012 NP-40 Lysis Buffer Beyotime CatP0013F jetPRIME Versatile DNA/siRNA Transfection Reagent Polyplus Cat#101000046 STAT3 Inhibitor VIII, 5,15-DPP Sigma-Aldrich Cat#573109 Recombinant Human Fibulin 5 Protein R&D Systems Cat#9006-FB-050 Critical commercial assays Annexin V-FITC Cell Apoptosis Detection Kit BD Biosciences Cat#556547 Reactive Oxygen Species Assay Kit Beyotime Cat#S0033M Cell Cycle and Apoptosis Analysis Kit Beyotime Cat#C1052 Cell Counting Kit-8 Beyotime Cat#C0038 BCA Protein Assay Kit Beyotime Cat#P0012 Lipid Peroxidation MDA Assay Kit Beyotime Cat#S0131M Total Exosome Isolation Reagent (from cell culture media) Thermo Fisher Scientific Cat#4478359 Dihydroethidium Thermo Fisher Scientific Cat#D11347 PKH67 Green Fluorescent Cell Linker Mini Kit Sigma-Aldrich Cat#MINI67 Dual-Luciferase® Reporter Assay System Promega, Madison, Wisconsin. .. Cat#E1910 AceQ Universal SYBR qPCR Master Mix Vazyme Cat#Q511-02 HiScript III RT SuperMix for qPCR (+gDNA wiper) Vazyme Cat#R323-01 Lipid Peroxidation MDA Assay Kit Beyotime Cat#S0130M ECL Western Blotting Substrate Thermo Fisher Scientific Cat#32106 Deposited data Digital spatial transcriptomics profiling This paper GSA: HRA010680 RNA-seq This paper GSA: HRA010129 MicroRNA seq This paper GSA: CRA022306 Single-cell RNA-seq This paper GSA: CRA022568 Experimental models: cell lines A549 ATCC Cat#CCL-185; RRID: CVCL_0023 MRC5 ATCC Cat#CCL-171; RRID: CVCL_0440 LLC ATCC Cat#CRL-1642; RRID: CVCL_4358 NIH-3T3 ATCC Cat#CRL-1658; RRID: CVCL_0594 (Continued on next page) Cell Reports 44, 116018, August 26, 2025 19
Sterility:Article Title: CAR-M2 immunotherapy resolves renal fibrosis via revascularization and apoptosis of profibrotic Cxcr2 + endothelial cells.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER SDS coolaber Cat#CS9701 Triton X-100 Solution (10%, Sterile) Beyotime CatST797 Tween 20 Beyotime Cat#ST825 DiR membrane dye probe Meilunbio Cat#MB12482 Dio membrane dye probe Meilunbio Cat#MB4239-1 Western and IP cell lysates Beyotime Cat#P0013 Protease inhibitor Mixture (generic, 100×) Beyotime Cat#P1005 Protease phosphatase inhibitor mixture Beyotime Cat#P1045 RIPA lysate Beyotime Cat#P0013B Loading buffer Solarbio Cat#D8371 PVDF membrane Merck millipore Cat#IPVH0010 Matrigel Corning Cat#354234 Goat serum Beyotime Cat#C0265 DAPI dye solution Beyotime Cat#C1005 Hoechst 33342 Mengbio Cat#MBR007 4% paraformaldehyde tissue fixative Biosharp Cat#BL539A Glycine Solaribio Cat#G8200 APS Solaribio Cat#A1010-500 10%SDS Solution Solaribio Cat#S1010 1M Tris-Hcl Buffer Solaribio Cat#T1020 1.5M Tris-Hcl Buffer Solaribio Cat#T1010 OCT frozen section embedding agent SAKURA Cat#4583 Methylprenylated hyaluronic acid EFL Cat#EFL-HAMA-150K Luciferase substrate Dowobio Cat#DW3113 Microfil Flow tech Inc Cat#MV-117 DNase I Roche Cat#10104159001 Recombinant Human Flt3-Ligand PeproTech Cat#300-19 Recombinant Human GM-CSF PeproTech Cat#300-03 Recombinant Human M-CSF PeproTech Cat#300-25 Recombinant Human TGF-β1 PeproTech Cat#100-21 SB265610 MACKLIN Cat#S921626 ZINC00640089 MedChemExpress Cat#667880-11-7 GM6001 aladdin Cat#G274767 Recombinant human MMP2 protein Solarbio Cat#P02344 ANG 193109 MedChemExpress Cat#HY-U00449 LG10268 MedChemExpress Cat#HY-15340 Corn oil MedChemExpress Cat#HY-Y1888 Critical commercial assays Mouse IL-4 ELISA Kit Beyotime Cat#PI612 Mouse IL-10 ELISA Kit Beyotime Cat#PI522 Mouse IFN-γ ELISA Kit Beyotime Cat#PI508 Modified Sirius red staining kit Solarbio Cat#G1472 Modified Masson staining kit Solarbio Cat#G1346 BCA protein concentration determination kit Beyotime Cat#P0012 TSA Fluorescence Triple Staining Kit ABclonal Cat#RK05903 Cell Counting Kit-8 Beyotime Cat#C0039 Annexin V-APC / PI Apoptosis Detection Kit Elabscience Cat#E-CK-A217 10× Genomics Chromium Genetic Analyzer 10× Genomics RRID:SCR_019326 (Continued on next page) e2 Cell Reports Medicine 7, 102698, April 21, 2026 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Biotinylated Human FAP Protein, His,AvitagTM (MALS verified) ACROBiosystems Cat#FAP-H82Q6 Spherotech Streptavidin Polystyrene Particles, 0.5%w/v, 5.0–5.9 μm Spherotech Cat#SVP-50-5 Alexa Fluor 488 NHS ESTER Invitrogen Cat#A20000 Agilent Bioanalyzer Agilent RRID:SCR_018043 Biological samples Human kidney tissue slice Department of Pathology at the Eighth Medical Center of Chinese PLA General Hospital N/A Deposited data scRNA-seq data of mice This paper GEO: GSE252943 (https://www. ncbi.nlm.nih.gov/geo/query/ acc.cgi?acc=GSE252943) scRNA-seq data of human Abedini et al.53 GEO: GSE211785 (https://www. ncbi.nlm.nih.gov/geo/query/ acc.cgi?acc=GSE211785) Bulk-RNA sequencing data This paper GEO: GSE282078 (https://www. ncbi.nlm.nih.gov/geo/query/ acc.cgi?acc=GSE282078) Experimental models: Cell lines Human Umbilical Venous Endothelial Cells (HUVEC) ATCC Cat#bio-81814 Primary human renal fibroblasts icellbioscience Cat#HUM-iCell-u013 THP-1 ATCC Cat#bio-73174 Mouse bone marrow-derived macrophages (BMDMs) This paper N/A hPSC-M Jun Shen lab (State key laboratory of Experimental Hematology)61 N/A Experimental models: Organisms/strains Mouse: balb/C Shanghai Model Organisms Center Cat#SM-003 Mouse: Rosa-LSL-RSR-tdTomato-DTR Shanghai Model Organisms Center N/A Mouse: Cxcr2-DreERT2 This paper N/A Mouse: Cdh5-CreERT2 Shanghai Model Organisms Center Cat#NM-KI-200173 Mouse: Cdh5-CreERT2; Cxcr2-DreERT2; Rosa-LSL-RSR-tdTomato-DTR This paper N/A Software and algorithms ImageJ https://imagej-nihgov.gate2.inist.fr/ij/ N/A FlowJo https://www.flowjo.com 10.8.1 Prism Graphpad https://www.graphpad.com/features Version10 Cell Ranger https://10xgenomics.com/ 7.0.1 R https://www.r-project.org/ 4.1.2 Seurat https://satijalab.org/seurat 5.0.1 Harmony https://github.com/immunogenomics/ harmony 0.1.1 msigdbr https://github.com/igordot/msigdbr 7.5.1 CellChat https://github.com/sqjin/CellChat 1.6.1 SCENIC https://github.com/aertslab/SCENIC 1.3.1 devtools https://CRAN.R-project.org/ package=devtools 2.4.5 ComplexHeatmap N/A 2.9.3 (Continued on next page) Cell Reports Medicine 7, 102698, April 21, 2026 e3 Article ll OPEN ACCESS
Western Blot:Article Title: CAR-M2 immunotherapy resolves renal fibrosis via revascularization and apoptosis of profibrotic Cxcr2 + endothelial cells.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER SDS coolaber Cat#CS9701 Triton X-100 Solution (10%, Sterile) Beyotime CatST797 Tween 20 Beyotime Cat#ST825 DiR membrane dye probe Meilunbio Cat#MB12482 Dio membrane dye probe Meilunbio Cat#MB4239-1 Western and IP cell lysates Beyotime Cat#P0013 Protease inhibitor Mixture (generic, 100×) Beyotime Cat#P1005 Protease phosphatase inhibitor mixture Beyotime Cat#P1045 RIPA lysate Beyotime Cat#P0013B Loading buffer Solarbio Cat#D8371 PVDF membrane Merck millipore Cat#IPVH0010 Matrigel Corning Cat#354234 Goat serum Beyotime Cat#C0265 DAPI dye solution Beyotime Cat#C1005 Hoechst 33342 Mengbio Cat#MBR007 4% paraformaldehyde tissue fixative Biosharp Cat#BL539A Glycine Solaribio Cat#G8200 APS Solaribio Cat#A1010-500 10%SDS Solution Solaribio Cat#S1010 1M Tris-Hcl Buffer Solaribio Cat#T1020 1.5M Tris-Hcl Buffer Solaribio Cat#T1010 OCT frozen section embedding agent SAKURA Cat#4583 Methylprenylated hyaluronic acid EFL Cat#EFL-HAMA-150K Luciferase substrate Dowobio Cat#DW3113 Microfil Flow tech Inc Cat#MV-117 DNase I Roche Cat#10104159001 Recombinant Human Flt3-Ligand PeproTech Cat#300-19 Recombinant Human GM-CSF PeproTech Cat#300-03 Recombinant Human M-CSF PeproTech Cat#300-25 Recombinant Human TGF-β1 PeproTech Cat#100-21 SB265610 MACKLIN Cat#S921626 ZINC00640089 MedChemExpress Cat#667880-11-7 GM6001 aladdin Cat#G274767 Recombinant human MMP2 protein Solarbio Cat#P02344 ANG 193109 MedChemExpress Cat#HY-U00449 LG10268 MedChemExpress Cat#HY-15340 Corn oil MedChemExpress Cat#HY-Y1888 Critical commercial assays Mouse IL-4 ELISA Kit Beyotime Cat#PI612 Mouse IL-10 ELISA Kit Beyotime Cat#PI522 Mouse IFN-γ ELISA Kit Beyotime Cat#PI508 Modified Sirius red staining kit Solarbio Cat#G1472 Modified Masson staining kit Solarbio Cat#G1346 BCA protein concentration determination kit Beyotime Cat#P0012 TSA Fluorescence Triple Staining Kit ABclonal Cat#RK05903 Cell Counting Kit-8 Beyotime Cat#C0039 Annexin V-APC / PI Apoptosis Detection Kit Elabscience Cat#E-CK-A217 10× Genomics Chromium Genetic Analyzer 10× Genomics RRID:SCR_019326 (Continued on next page) e2 Cell Reports Medicine 7, 102698, April 21, 2026 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Biotinylated Human FAP Protein, His,AvitagTM (MALS verified) ACROBiosystems Cat#FAP-H82Q6 Spherotech Streptavidin Polystyrene Particles, 0.5%w/v, 5.0–5.9 μm Spherotech Cat#SVP-50-5 Alexa Fluor 488 NHS ESTER Invitrogen Cat#A20000 Agilent Bioanalyzer Agilent RRID:SCR_018043 Biological samples Human kidney tissue slice Department of Pathology at the Eighth Medical Center of Chinese PLA General Hospital N/A Deposited data scRNA-seq data of mice This paper GEO: GSE252943 (https://www. ncbi.nlm.nih.gov/geo/query/ acc.cgi?acc=GSE252943) scRNA-seq data of human Abedini et al.53 GEO: GSE211785 (https://www. ncbi.nlm.nih.gov/geo/query/ acc.cgi?acc=GSE211785) Bulk-RNA sequencing data This paper GEO: GSE282078 (https://www. ncbi.nlm.nih.gov/geo/query/ acc.cgi?acc=GSE282078) Experimental models: Cell lines Human Umbilical Venous Endothelial Cells (HUVEC) ATCC Cat#bio-81814 Primary human renal fibroblasts icellbioscience Cat#HUM-iCell-u013 THP-1 ATCC Cat#bio-73174 Mouse bone marrow-derived macrophages (BMDMs) This paper N/A hPSC-M Jun Shen lab (State key laboratory of Experimental Hematology)61 N/A Experimental models: Organisms/strains Mouse: balb/C Shanghai Model Organisms Center Cat#SM-003 Mouse: Rosa-LSL-RSR-tdTomato-DTR Shanghai Model Organisms Center N/A Mouse: Cxcr2-DreERT2 This paper N/A Mouse: Cdh5-CreERT2 Shanghai Model Organisms Center Cat#NM-KI-200173 Mouse: Cdh5-CreERT2; Cxcr2-DreERT2; Rosa-LSL-RSR-tdTomato-DTR This paper N/A Software and algorithms ImageJ https://imagej-nihgov.gate2.inist.fr/ij/ N/A FlowJo https://www.flowjo.com 10.8.1 Prism Graphpad https://www.graphpad.com/features Version10 Cell Ranger https://10xgenomics.com/ 7.0.1 R https://www.r-project.org/ 4.1.2 Seurat https://satijalab.org/seurat 5.0.1 Harmony https://github.com/immunogenomics/ harmony 0.1.1 msigdbr https://github.com/igordot/msigdbr 7.5.1 CellChat https://github.com/sqjin/CellChat 1.6.1 SCENIC https://github.com/aertslab/SCENIC 1.3.1 devtools https://CRAN.R-project.org/ package=devtools 2.4.5 ComplexHeatmap N/A 2.9.3 (Continued on next page) Cell Reports Medicine 7, 102698, April 21, 2026 e3 Article ll OPEN ACCESS
Protease Inhibitor:Article Title: CAR-M2 immunotherapy resolves renal fibrosis via revascularization and apoptosis of profibrotic Cxcr2 + endothelial cells.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER SDS coolaber Cat#CS9701 Triton X-100 Solution (10%, Sterile) Beyotime CatST797 Tween 20 Beyotime Cat#ST825 DiR membrane dye probe Meilunbio Cat#MB12482 Dio membrane dye probe Meilunbio Cat#MB4239-1 Western and IP cell lysates Beyotime Cat#P0013 Protease inhibitor Mixture (generic, 100×) Beyotime Cat#P1005 Protease phosphatase inhibitor mixture Beyotime Cat#P1045 RIPA lysate Beyotime Cat#P0013B Loading buffer Solarbio Cat#D8371 PVDF membrane Merck millipore Cat#IPVH0010 Matrigel Corning Cat#354234 Goat serum Beyotime Cat#C0265 DAPI dye solution Beyotime Cat#C1005 Hoechst 33342 Mengbio Cat#MBR007 4% paraformaldehyde tissue fixative Biosharp Cat#BL539A Glycine Solaribio Cat#G8200 APS Solaribio Cat#A1010-500 10%SDS Solution Solaribio Cat#S1010 1M Tris-Hcl Buffer Solaribio Cat#T1020 1.5M Tris-Hcl Buffer Solaribio Cat#T1010 OCT frozen section embedding agent SAKURA Cat#4583 Methylprenylated hyaluronic acid EFL Cat#EFL-HAMA-150K Luciferase substrate Dowobio Cat#DW3113 Microfil Flow tech Inc Cat#MV-117 DNase I Roche Cat#10104159001 Recombinant Human Flt3-Ligand PeproTech Cat#300-19 Recombinant Human GM-CSF PeproTech Cat#300-03 Recombinant Human M-CSF PeproTech Cat#300-25 Recombinant Human TGF-β1 PeproTech Cat#100-21 SB265610 MACKLIN Cat#S921626 ZINC00640089 MedChemExpress Cat#667880-11-7 GM6001 aladdin Cat#G274767 Recombinant human MMP2 protein Solarbio Cat#P02344 ANG 193109 MedChemExpress Cat#HY-U00449 LG10268 MedChemExpress Cat#HY-15340 Corn oil MedChemExpress Cat#HY-Y1888 Critical commercial assays Mouse IL-4 ELISA Kit Beyotime Cat#PI612 Mouse IL-10 ELISA Kit Beyotime Cat#PI522 Mouse IFN-γ ELISA Kit Beyotime Cat#PI508 Modified Sirius red staining kit Solarbio Cat#G1472 Modified Masson staining kit Solarbio Cat#G1346 BCA protein concentration determination kit Beyotime Cat#P0012 TSA Fluorescence Triple Staining Kit ABclonal Cat#RK05903 Cell Counting Kit-8 Beyotime Cat#C0039 Annexin V-APC / PI Apoptosis Detection Kit Elabscience Cat#E-CK-A217 10× Genomics Chromium Genetic Analyzer 10× Genomics RRID:SCR_019326 (Continued on next page) e2 Cell Reports Medicine 7, 102698, April 21, 2026 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Biotinylated Human FAP Protein, His,AvitagTM (MALS verified) ACROBiosystems Cat#FAP-H82Q6 Spherotech Streptavidin Polystyrene Particles, 0.5%w/v, 5.0–5.9 μm Spherotech Cat#SVP-50-5 Alexa Fluor 488 NHS ESTER Invitrogen Cat#A20000 Agilent Bioanalyzer Agilent RRID:SCR_018043 Biological samples Human kidney tissue slice Department of Pathology at the Eighth Medical Center of Chinese PLA General Hospital N/A Deposited data scRNA-seq data of mice This paper GEO: GSE252943 (https://www. ncbi.nlm.nih.gov/geo/query/ acc.cgi?acc=GSE252943) scRNA-seq data of human Abedini et al.53 GEO: GSE211785 (https://www. ncbi.nlm.nih.gov/geo/query/ acc.cgi?acc=GSE211785) Bulk-RNA sequencing data This paper GEO: GSE282078 (https://www. ncbi.nlm.nih.gov/geo/query/ acc.cgi?acc=GSE282078) Experimental models: Cell lines Human Umbilical Venous Endothelial Cells (HUVEC) ATCC Cat#bio-81814 Primary human renal fibroblasts icellbioscience Cat#HUM-iCell-u013 THP-1 ATCC Cat#bio-73174 Mouse bone marrow-derived macrophages (BMDMs) This paper N/A hPSC-M Jun Shen lab (State key laboratory of Experimental Hematology)61 N/A Experimental models: Organisms/strains Mouse: balb/C Shanghai Model Organisms Center Cat#SM-003 Mouse: Rosa-LSL-RSR-tdTomato-DTR Shanghai Model Organisms Center N/A Mouse: Cxcr2-DreERT2 This paper N/A Mouse: Cdh5-CreERT2 Shanghai Model Organisms Center Cat#NM-KI-200173 Mouse: Cdh5-CreERT2; Cxcr2-DreERT2; Rosa-LSL-RSR-tdTomato-DTR This paper N/A Software and algorithms ImageJ https://imagej-nihgov.gate2.inist.fr/ij/ N/A FlowJo https://www.flowjo.com 10.8.1 Prism Graphpad https://www.graphpad.com/features Version10 Cell Ranger https://10xgenomics.com/ 7.0.1 R https://www.r-project.org/ 4.1.2 Seurat https://satijalab.org/seurat 5.0.1 Harmony https://github.com/immunogenomics/ harmony 0.1.1 msigdbr https://github.com/igordot/msigdbr 7.5.1 CellChat https://github.com/sqjin/CellChat 1.6.1 SCENIC https://github.com/aertslab/SCENIC 1.3.1 devtools https://CRAN.R-project.org/ package=devtools 2.4.5 ComplexHeatmap N/A 2.9.3 (Continued on next page) Cell Reports Medicine 7, 102698, April 21, 2026 e3 Article ll OPEN ACCESS
Luciferase:Article Title: CAR-M2 immunotherapy resolves renal fibrosis via revascularization and apoptosis of profibrotic Cxcr2 + endothelial cells.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER SDS coolaber Cat#CS9701 Triton X-100 Solution (10%, Sterile) Beyotime CatST797 Tween 20 Beyotime Cat#ST825 DiR membrane dye probe Meilunbio Cat#MB12482 Dio membrane dye probe Meilunbio Cat#MB4239-1 Western and IP cell lysates Beyotime Cat#P0013 Protease inhibitor Mixture (generic, 100×) Beyotime Cat#P1005 Protease phosphatase inhibitor mixture Beyotime Cat#P1045 RIPA lysate Beyotime Cat#P0013B Loading buffer Solarbio Cat#D8371 PVDF membrane Merck millipore Cat#IPVH0010 Matrigel Corning Cat#354234 Goat serum Beyotime Cat#C0265 DAPI dye solution Beyotime Cat#C1005 Hoechst 33342 Mengbio Cat#MBR007 4% paraformaldehyde tissue fixative Biosharp Cat#BL539A Glycine Solaribio Cat#G8200 APS Solaribio Cat#A1010-500 10%SDS Solution Solaribio Cat#S1010 1M Tris-Hcl Buffer Solaribio Cat#T1020 1.5M Tris-Hcl Buffer Solaribio Cat#T1010 OCT frozen section embedding agent SAKURA Cat#4583 Methylprenylated hyaluronic acid EFL Cat#EFL-HAMA-150K Luciferase substrate Dowobio Cat#DW3113 Microfil Flow tech Inc Cat#MV-117 DNase I Roche Cat#10104159001 Recombinant Human Flt3-Ligand PeproTech Cat#300-19 Recombinant Human GM-CSF PeproTech Cat#300-03 Recombinant Human M-CSF PeproTech Cat#300-25 Recombinant Human TGF-β1 PeproTech Cat#100-21 SB265610 MACKLIN Cat#S921626 ZINC00640089 MedChemExpress Cat#667880-11-7 GM6001 aladdin Cat#G274767 Recombinant human MMP2 protein Solarbio Cat#P02344 ANG 193109 MedChemExpress Cat#HY-U00449 LG10268 MedChemExpress Cat#HY-15340 Corn oil MedChemExpress Cat#HY-Y1888 Critical commercial assays Mouse IL-4 ELISA Kit Beyotime Cat#PI612 Mouse IL-10 ELISA Kit Beyotime Cat#PI522 Mouse IFN-γ ELISA Kit Beyotime Cat#PI508 Modified Sirius red staining kit Solarbio Cat#G1472 Modified Masson staining kit Solarbio Cat#G1346 BCA protein concentration determination kit Beyotime Cat#P0012 TSA Fluorescence Triple Staining Kit ABclonal Cat#RK05903 Cell Counting Kit-8 Beyotime Cat#C0039 Annexin V-APC / PI Apoptosis Detection Kit Elabscience Cat#E-CK-A217 10× Genomics Chromium Genetic Analyzer 10× Genomics RRID:SCR_019326 (Continued on next page) e2 Cell Reports Medicine 7, 102698, April 21, 2026 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Biotinylated Human FAP Protein, His,AvitagTM (MALS verified) ACROBiosystems Cat#FAP-H82Q6 Spherotech Streptavidin Polystyrene Particles, 0.5%w/v, 5.0–5.9 μm Spherotech Cat#SVP-50-5 Alexa Fluor 488 NHS ESTER Invitrogen Cat#A20000 Agilent Bioanalyzer Agilent RRID:SCR_018043 Biological samples Human kidney tissue slice Department of Pathology at the Eighth Medical Center of Chinese PLA General Hospital N/A Deposited data scRNA-seq data of mice This paper GEO: GSE252943 (https://www. ncbi.nlm.nih.gov/geo/query/ acc.cgi?acc=GSE252943) scRNA-seq data of human Abedini et al.53 GEO: GSE211785 (https://www. ncbi.nlm.nih.gov/geo/query/ acc.cgi?acc=GSE211785) Bulk-RNA sequencing data This paper GEO: GSE282078 (https://www. ncbi.nlm.nih.gov/geo/query/ acc.cgi?acc=GSE282078) Experimental models: Cell lines Human Umbilical Venous Endothelial Cells (HUVEC) ATCC Cat#bio-81814 Primary human renal fibroblasts icellbioscience Cat#HUM-iCell-u013 THP-1 ATCC Cat#bio-73174 Mouse bone marrow-derived macrophages (BMDMs) This paper N/A hPSC-M Jun Shen lab (State key laboratory of Experimental Hematology)61 N/A Experimental models: Organisms/strains Mouse: balb/C Shanghai Model Organisms Center Cat#SM-003 Mouse: Rosa-LSL-RSR-tdTomato-DTR Shanghai Model Organisms Center N/A Mouse: Cxcr2-DreERT2 This paper N/A Mouse: Cdh5-CreERT2 Shanghai Model Organisms Center Cat#NM-KI-200173 Mouse: Cdh5-CreERT2; Cxcr2-DreERT2; Rosa-LSL-RSR-tdTomato-DTR This paper N/A Software and algorithms ImageJ https://imagej-nihgov.gate2.inist.fr/ij/ N/A FlowJo https://www.flowjo.com 10.8.1 Prism Graphpad https://www.graphpad.com/features Version10 Cell Ranger https://10xgenomics.com/ 7.0.1 R https://www.r-project.org/ 4.1.2 Seurat https://satijalab.org/seurat 5.0.1 Harmony https://github.com/immunogenomics/ harmony 0.1.1 msigdbr https://github.com/igordot/msigdbr 7.5.1 CellChat https://github.com/sqjin/CellChat 1.6.1 SCENIC https://github.com/aertslab/SCENIC 1.3.1 devtools https://CRAN.R-project.org/ package=devtools 2.4.5 ComplexHeatmap N/A 2.9.3 (Continued on next page) Cell Reports Medicine 7, 102698, April 21, 2026 e3 Article ll OPEN ACCESS
Modification:Article Title: CAR-M2 immunotherapy resolves renal fibrosis via revascularization and apoptosis of profibrotic Cxcr2 + endothelial cells.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER SDS coolaber Cat#CS9701 Triton X-100 Solution (10%, Sterile) Beyotime CatST797 Tween 20 Beyotime Cat#ST825 DiR membrane dye probe Meilunbio Cat#MB12482 Dio membrane dye probe Meilunbio Cat#MB4239-1 Western and IP cell lysates Beyotime Cat#P0013 Protease inhibitor Mixture (generic, 100×) Beyotime Cat#P1005 Protease phosphatase inhibitor mixture Beyotime Cat#P1045 RIPA lysate Beyotime Cat#P0013B Loading buffer Solarbio Cat#D8371 PVDF membrane Merck millipore Cat#IPVH0010 Matrigel Corning Cat#354234 Goat serum Beyotime Cat#C0265 DAPI dye solution Beyotime Cat#C1005 Hoechst 33342 Mengbio Cat#MBR007 4% paraformaldehyde tissue fixative Biosharp Cat#BL539A Glycine Solaribio Cat#G8200 APS Solaribio Cat#A1010-500 10%SDS Solution Solaribio Cat#S1010 1M Tris-Hcl Buffer Solaribio Cat#T1020 1.5M Tris-Hcl Buffer Solaribio Cat#T1010 OCT frozen section embedding agent SAKURA Cat#4583 Methylprenylated hyaluronic acid EFL Cat#EFL-HAMA-150K Luciferase substrate Dowobio Cat#DW3113 Microfil Flow tech Inc Cat#MV-117 DNase I Roche Cat#10104159001 Recombinant Human Flt3-Ligand PeproTech Cat#300-19 Recombinant Human GM-CSF PeproTech Cat#300-03 Recombinant Human M-CSF PeproTech Cat#300-25 Recombinant Human TGF-β1 PeproTech Cat#100-21 SB265610 MACKLIN Cat#S921626 ZINC00640089 MedChemExpress Cat#667880-11-7 GM6001 aladdin Cat#G274767 Recombinant human MMP2 protein Solarbio Cat#P02344 ANG 193109 MedChemExpress Cat#HY-U00449 LG10268 MedChemExpress Cat#HY-15340 Corn oil MedChemExpress Cat#HY-Y1888 Critical commercial assays Mouse IL-4 ELISA Kit Beyotime Cat#PI612 Mouse IL-10 ELISA Kit Beyotime Cat#PI522 Mouse IFN-γ ELISA Kit Beyotime Cat#PI508 Modified Sirius red staining kit Solarbio Cat#G1472 Modified Masson staining kit Solarbio Cat#G1346 BCA protein concentration determination kit Beyotime Cat#P0012 TSA Fluorescence Triple Staining Kit ABclonal Cat#RK05903 Cell Counting Kit-8 Beyotime Cat#C0039 Annexin V-APC / PI Apoptosis Detection Kit Elabscience Cat#E-CK-A217 10× Genomics Chromium Genetic Analyzer 10× Genomics RRID:SCR_019326 (Continued on next page) e2 Cell Reports Medicine 7, 102698, April 21, 2026 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Biotinylated Human FAP Protein, His,AvitagTM (MALS verified) ACROBiosystems Cat#FAP-H82Q6 Spherotech Streptavidin Polystyrene Particles, 0.5%w/v, 5.0–5.9 μm Spherotech Cat#SVP-50-5 Alexa Fluor 488 NHS ESTER Invitrogen Cat#A20000 Agilent Bioanalyzer Agilent RRID:SCR_018043 Biological samples Human kidney tissue slice Department of Pathology at the Eighth Medical Center of Chinese PLA General Hospital N/A Deposited data scRNA-seq data of mice This paper GEO: GSE252943 (https://www. ncbi.nlm.nih.gov/geo/query/ acc.cgi?acc=GSE252943) scRNA-seq data of human Abedini et al.53 GEO: GSE211785 (https://www. ncbi.nlm.nih.gov/geo/query/ acc.cgi?acc=GSE211785) Bulk-RNA sequencing data This paper GEO: GSE282078 (https://www. ncbi.nlm.nih.gov/geo/query/ acc.cgi?acc=GSE282078) Experimental models: Cell lines Human Umbilical Venous Endothelial Cells (HUVEC) ATCC Cat#bio-81814 Primary human renal fibroblasts icellbioscience Cat#HUM-iCell-u013 THP-1 ATCC Cat#bio-73174 Mouse bone marrow-derived macrophages (BMDMs) This paper N/A hPSC-M Jun Shen lab (State key laboratory of Experimental Hematology)61 N/A Experimental models: Organisms/strains Mouse: balb/C Shanghai Model Organisms Center Cat#SM-003 Mouse: Rosa-LSL-RSR-tdTomato-DTR Shanghai Model Organisms Center N/A Mouse: Cxcr2-DreERT2 This paper N/A Mouse: Cdh5-CreERT2 Shanghai Model Organisms Center Cat#NM-KI-200173 Mouse: Cdh5-CreERT2; Cxcr2-DreERT2; Rosa-LSL-RSR-tdTomato-DTR This paper N/A Software and algorithms ImageJ https://imagej-nihgov.gate2.inist.fr/ij/ N/A FlowJo https://www.flowjo.com 10.8.1 Prism Graphpad https://www.graphpad.com/features Version10 Cell Ranger https://10xgenomics.com/ 7.0.1 R https://www.r-project.org/ 4.1.2 Seurat https://satijalab.org/seurat 5.0.1 Harmony https://github.com/immunogenomics/ harmony 0.1.1 msigdbr https://github.com/igordot/msigdbr 7.5.1 CellChat https://github.com/sqjin/CellChat 1.6.1 SCENIC https://github.com/aertslab/SCENIC 1.3.1 devtools https://CRAN.R-project.org/ package=devtools 2.4.5 ComplexHeatmap N/A 2.9.3 (Continued on next page) Cell Reports Medicine 7, 102698, April 21, 2026 e3 Article ll OPEN ACCESS
Staining:Article Title: CAR-M2 immunotherapy resolves renal fibrosis via revascularization and apoptosis of profibrotic Cxcr2 + endothelial cells.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER SDS coolaber Cat#CS9701 Triton X-100 Solution (10%, Sterile) Beyotime CatST797 Tween 20 Beyotime Cat#ST825 DiR membrane dye probe Meilunbio Cat#MB12482 Dio membrane dye probe Meilunbio Cat#MB4239-1 Western and IP cell lysates Beyotime Cat#P0013 Protease inhibitor Mixture (generic, 100×) Beyotime Cat#P1005 Protease phosphatase inhibitor mixture Beyotime Cat#P1045 RIPA lysate Beyotime Cat#P0013B Loading buffer Solarbio Cat#D8371 PVDF membrane Merck millipore Cat#IPVH0010 Matrigel Corning Cat#354234 Goat serum Beyotime Cat#C0265 DAPI dye solution Beyotime Cat#C1005 Hoechst 33342 Mengbio Cat#MBR007 4% paraformaldehyde tissue fixative Biosharp Cat#BL539A Glycine Solaribio Cat#G8200 APS Solaribio Cat#A1010-500 10%SDS Solution Solaribio Cat#S1010 1M Tris-Hcl Buffer Solaribio Cat#T1020 1.5M Tris-Hcl Buffer Solaribio Cat#T1010 OCT frozen section embedding agent SAKURA Cat#4583 Methylprenylated hyaluronic acid EFL Cat#EFL-HAMA-150K Luciferase substrate Dowobio Cat#DW3113 Microfil Flow tech Inc Cat#MV-117 DNase I Roche Cat#10104159001 Recombinant Human Flt3-Ligand PeproTech Cat#300-19 Recombinant Human GM-CSF PeproTech Cat#300-03 Recombinant Human M-CSF PeproTech Cat#300-25 Recombinant Human TGF-β1 PeproTech Cat#100-21 SB265610 MACKLIN Cat#S921626 ZINC00640089 MedChemExpress Cat#667880-11-7 GM6001 aladdin Cat#G274767 Recombinant human MMP2 protein Solarbio Cat#P02344 ANG 193109 MedChemExpress Cat#HY-U00449 LG10268 MedChemExpress Cat#HY-15340 Corn oil MedChemExpress Cat#HY-Y1888 Critical commercial assays Mouse IL-4 ELISA Kit Beyotime Cat#PI612 Mouse IL-10 ELISA Kit Beyotime Cat#PI522 Mouse IFN-γ ELISA Kit Beyotime Cat#PI508 Modified Sirius red staining kit Solarbio Cat#G1472 Modified Masson staining kit Solarbio Cat#G1346 BCA protein concentration determination kit Beyotime Cat#P0012 TSA Fluorescence Triple Staining Kit ABclonal Cat#RK05903 Cell Counting Kit-8 Beyotime Cat#C0039 Annexin V-APC / PI Apoptosis Detection Kit Elabscience Cat#E-CK-A217 10× Genomics Chromium Genetic Analyzer 10× Genomics RRID:SCR_019326 (Continued on next page) e2 Cell Reports Medicine 7, 102698, April 21, 2026 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Biotinylated Human FAP Protein, His,AvitagTM (MALS verified) ACROBiosystems Cat#FAP-H82Q6 Spherotech Streptavidin Polystyrene Particles, 0.5%w/v, 5.0–5.9 μm Spherotech Cat#SVP-50-5 Alexa Fluor 488 NHS ESTER Invitrogen Cat#A20000 Agilent Bioanalyzer Agilent RRID:SCR_018043 Biological samples Human kidney tissue slice Department of Pathology at the Eighth Medical Center of Chinese PLA General Hospital N/A Deposited data scRNA-seq data of mice This paper GEO: GSE252943 (https://www. ncbi.nlm.nih.gov/geo/query/ acc.cgi?acc=GSE252943) scRNA-seq data of human Abedini et al.53 GEO: GSE211785 (https://www. ncbi.nlm.nih.gov/geo/query/ acc.cgi?acc=GSE211785) Bulk-RNA sequencing data This paper GEO: GSE282078 (https://www. ncbi.nlm.nih.gov/geo/query/ acc.cgi?acc=GSE282078) Experimental models: Cell lines Human Umbilical Venous Endothelial Cells (HUVEC) ATCC Cat#bio-81814 Primary human renal fibroblasts icellbioscience Cat#HUM-iCell-u013 THP-1 ATCC Cat#bio-73174 Mouse bone marrow-derived macrophages (BMDMs) This paper N/A hPSC-M Jun Shen lab (State key laboratory of Experimental Hematology)61 N/A Experimental models: Organisms/strains Mouse: balb/C Shanghai Model Organisms Center Cat#SM-003 Mouse: Rosa-LSL-RSR-tdTomato-DTR Shanghai Model Organisms Center N/A Mouse: Cxcr2-DreERT2 This paper N/A Mouse: Cdh5-CreERT2 Shanghai Model Organisms Center Cat#NM-KI-200173 Mouse: Cdh5-CreERT2; Cxcr2-DreERT2; Rosa-LSL-RSR-tdTomato-DTR This paper N/A Software and algorithms ImageJ https://imagej-nihgov.gate2.inist.fr/ij/ N/A FlowJo https://www.flowjo.com 10.8.1 Prism Graphpad https://www.graphpad.com/features Version10 Cell Ranger https://10xgenomics.com/ 7.0.1 R https://www.r-project.org/ 4.1.2 Seurat https://satijalab.org/seurat 5.0.1 Harmony https://github.com/immunogenomics/ harmony 0.1.1 msigdbr https://github.com/igordot/msigdbr 7.5.1 CellChat https://github.com/sqjin/CellChat 1.6.1 SCENIC https://github.com/aertslab/SCENIC 1.3.1 devtools https://CRAN.R-project.org/ package=devtools 2.4.5 ComplexHeatmap N/A 2.9.3 (Continued on next page) Cell Reports Medicine 7, 102698, April 21, 2026 e3 Article ll OPEN ACCESS
Article Title: METTL3 regulates α-KG-dependent mitophagy and apoptosis in nucleus pulposus cells through the MALAT1/miR-23c/IDH1 axis.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-ACAN Affinity Cat#DF7561; RRID: AB_2841055 Rabbit anti-COLL II Proteintech Cat#28459-1-AP; RRID: AB_2881147 Rabbit anti-MMP13 Proteintech Cat#18165-1-AP; RRID: AB_2144858 Rabbit anti-ADAMTS5 Affinity Cat#DF13268; RRID: AB_2846287 Rabbit anti-GAPDH Proteintech Cat#60004-1-Ig; RRID: AB_2107436 Rabbit anti-IL-6 Abcam Cat#ab233706; RRID: AB_2889391 Rabbit anti-TNF-α Abcam Cat#ab183218; RRID: AB_2889388 Rabbit anti-Cleaved PARP-1 ThermoFisher Cat#44-698G; RRID: AB_2533726 Rabbit anti-Cleaved Caspase-3 Cell Signaling Technology Cat#9661; RRID: AB_2341188 Rabbit anti-BAX Proteintech Cat#50599-2; RRID: AB_2061561 Rabbit anti-BCL2 Affinity Cat#AF6139; RRID: AB_2835021 Rabbit anti-CAT Abclonal Cat#A11777; RRID: AB_2758747 Rabbit anti-SOD1 Abcam Cat#ab13498; RRID: AB_300402 Rabbit anti-p62 Proteintech Cat#18420-1-AP; RRID: AB_10694431 Rabbit anti-LC3B-II Cell Signaling Technology Cat#2775S; RRID: AB_915950 Rabbit anti-PINK1 Abcam Cat#ab216144; RRID: AB_2927726 Rabbit anti-Parkin Abcam Cat#ab315376; RRID: AB_3668762 Rabbit anti-p-Parkin Thermo Fisher Cat#PA5-114616; RRID: AB_2899252 Rabbit anti-Ubiquitin-Ser65 Boston Biochem Cat#A-110; RRID: AB_2636932 Rabbit anti-IDH1 Abcam Cat#ab256557; RRID: AB_3099653 Rabbit anti-IDH2 Abcam Cat#ab131263; RRID: AB_11156098 Rabbit anti-METTL3 Cell Signaling Technology Cat#86132; RRID: AB_2800072 Biological samples Human nucleus pulposus tissue This paper N/A HEK 293T cells ATCC Cat# CRL-11268 Chemicals, peptides, and recombinant proteins Type II collagenase Invitrogen Cat#17101015 DMEM/F12 medium Gibco Cat#11320033 Fetal bovine serum Gibco Cat#10099141C Penicillin/streptomycin Gibco Cat#15140122 TRIzol reagent Invitrogen Cat15596018CN RIPA Lysis Buffer Beyotime Cat#P0013B Phenylmethanesulfonyl fluoride Beyotime Cat#ST505 DAPI Solarbio Cat#C0065 Actinomycin D MedChem Cat#HY-17559 QuickBlock TM Blocking Buffer Beyotime Cat#P0220 Lipofectamine TM 2000 Invitrogen Cat#11668019 Critical commercial assays α-KG Assay Kit GraceBiotechnology Cat#G0861W Cell Counting Kit-8 Beyotime Cat#C0037 Senescence β-Galactosidase Staining Kit Beyotime Cat#C0602 EdU Cell Proliferation Kit with Alexa Fluor 594 Beyotime Cat#C0078S BCA Protein Assay Kit Beyotime Cat#P0011 (Continued on next page) 20 Cell Reports 45, 116793, January 27, 2026 .. Small pieces of NP tissue that were intraoperatively collected were subjected to 8 h of digestion with 0.2% concentration of type II collagenase (Invitrogen, USA) at 37 ◦ C, followed by 5 min of centrifugation at 1200 rpm.
Article Title: NSD2 drives triple-negative breast cancer progression via ATAD3A-dependent senescence.
Article Snippet: Triple-negative breast cancer (TNBC) represents the most aggressive subtype of breast cancer, with limited therapeutic options and dismal prognosis.. Histone methyltransferase NSD2 and its catalytic product H3K36me2 are established drivers of TNBC metastasis; however, the downstream effectors linking epigenetic regulation to cellular senescence remain largely unknown.. This study utilized transcriptomic profiling, ChIP-qPCR, and in vivo xenograft models to define the function and molecular mechanism of the NSD2-ATAD3A axis.
Protein Concentration:Article Title: CAR-M2 immunotherapy resolves renal fibrosis via revascularization and apoptosis of profibrotic Cxcr2 + endothelial cells.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER SDS coolaber Cat#CS9701 Triton X-100 Solution (10%, Sterile) Beyotime CatST797 Tween 20 Beyotime Cat#ST825 DiR membrane dye probe Meilunbio Cat#MB12482 Dio membrane dye probe Meilunbio Cat#MB4239-1 Western and IP cell lysates Beyotime Cat#P0013 Protease inhibitor Mixture (generic, 100×) Beyotime Cat#P1005 Protease phosphatase inhibitor mixture Beyotime Cat#P1045 RIPA lysate Beyotime Cat#P0013B Loading buffer Solarbio Cat#D8371 PVDF membrane Merck millipore Cat#IPVH0010 Matrigel Corning Cat#354234 Goat serum Beyotime Cat#C0265 DAPI dye solution Beyotime Cat#C1005 Hoechst 33342 Mengbio Cat#MBR007 4% paraformaldehyde tissue fixative Biosharp Cat#BL539A Glycine Solaribio Cat#G8200 APS Solaribio Cat#A1010-500 10%SDS Solution Solaribio Cat#S1010 1M Tris-Hcl Buffer Solaribio Cat#T1020 1.5M Tris-Hcl Buffer Solaribio Cat#T1010 OCT frozen section embedding agent SAKURA Cat#4583 Methylprenylated hyaluronic acid EFL Cat#EFL-HAMA-150K Luciferase substrate Dowobio Cat#DW3113 Microfil Flow tech Inc Cat#MV-117 DNase I Roche Cat#10104159001 Recombinant Human Flt3-Ligand PeproTech Cat#300-19 Recombinant Human GM-CSF PeproTech Cat#300-03 Recombinant Human M-CSF PeproTech Cat#300-25 Recombinant Human TGF-β1 PeproTech Cat#100-21 SB265610 MACKLIN Cat#S921626 ZINC00640089 MedChemExpress Cat#667880-11-7 GM6001 aladdin Cat#G274767 Recombinant human MMP2 protein Solarbio Cat#P02344 ANG 193109 MedChemExpress Cat#HY-U00449 LG10268 MedChemExpress Cat#HY-15340 Corn oil MedChemExpress Cat#HY-Y1888 Critical commercial assays Mouse IL-4 ELISA Kit Beyotime Cat#PI612 Mouse IL-10 ELISA Kit Beyotime Cat#PI522 Mouse IFN-γ ELISA Kit Beyotime Cat#PI508 Modified Sirius red staining kit Solarbio Cat#G1472 Modified Masson staining kit Solarbio Cat#G1346 BCA protein concentration determination kit Beyotime Cat#P0012 TSA Fluorescence Triple Staining Kit ABclonal Cat#RK05903 Cell Counting Kit-8 Beyotime Cat#C0039 Annexin V-APC / PI Apoptosis Detection Kit Elabscience Cat#E-CK-A217 10× Genomics Chromium Genetic Analyzer 10× Genomics RRID:SCR_019326 (Continued on next page) e2 Cell Reports Medicine 7, 102698, April 21, 2026 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Biotinylated Human FAP Protein, His,AvitagTM (MALS verified) ACROBiosystems Cat#FAP-H82Q6 Spherotech Streptavidin Polystyrene Particles, 0.5%w/v, 5.0–5.9 μm Spherotech Cat#SVP-50-5 Alexa Fluor 488 NHS ESTER Invitrogen Cat#A20000 Agilent Bioanalyzer Agilent RRID:SCR_018043 Biological samples Human kidney tissue slice Department of Pathology at the Eighth Medical Center of Chinese PLA General Hospital N/A Deposited data scRNA-seq data of mice This paper GEO: GSE252943 (https://www. ncbi.nlm.nih.gov/geo/query/ acc.cgi?acc=GSE252943) scRNA-seq data of human Abedini et al.53 GEO: GSE211785 (https://www. ncbi.nlm.nih.gov/geo/query/ acc.cgi?acc=GSE211785) Bulk-RNA sequencing data This paper GEO: GSE282078 (https://www. ncbi.nlm.nih.gov/geo/query/ acc.cgi?acc=GSE282078) Experimental models: Cell lines Human Umbilical Venous Endothelial Cells (HUVEC) ATCC Cat#bio-81814 Primary human renal fibroblasts icellbioscience Cat#HUM-iCell-u013 THP-1 ATCC Cat#bio-73174 Mouse bone marrow-derived macrophages (BMDMs) This paper N/A hPSC-M Jun Shen lab (State key laboratory of Experimental Hematology)61 N/A Experimental models: Organisms/strains Mouse: balb/C Shanghai Model Organisms Center Cat#SM-003 Mouse: Rosa-LSL-RSR-tdTomato-DTR Shanghai Model Organisms Center N/A Mouse: Cxcr2-DreERT2 This paper N/A Mouse: Cdh5-CreERT2 Shanghai Model Organisms Center Cat#NM-KI-200173 Mouse: Cdh5-CreERT2; Cxcr2-DreERT2; Rosa-LSL-RSR-tdTomato-DTR This paper N/A Software and algorithms ImageJ https://imagej-nihgov.gate2.inist.fr/ij/ N/A FlowJo https://www.flowjo.com 10.8.1 Prism Graphpad https://www.graphpad.com/features Version10 Cell Ranger https://10xgenomics.com/ 7.0.1 R https://www.r-project.org/ 4.1.2 Seurat https://satijalab.org/seurat 5.0.1 Harmony https://github.com/immunogenomics/ harmony 0.1.1 msigdbr https://github.com/igordot/msigdbr 7.5.1 CellChat https://github.com/sqjin/CellChat 1.6.1 SCENIC https://github.com/aertslab/SCENIC 1.3.1 devtools https://CRAN.R-project.org/ package=devtools 2.4.5 ComplexHeatmap N/A 2.9.3 (Continued on next page) Cell Reports Medicine 7, 102698, April 21, 2026 e3 Article ll OPEN ACCESS
Fluorescence:Article Title: CAR-M2 immunotherapy resolves renal fibrosis via revascularization and apoptosis of profibrotic Cxcr2 + endothelial cells.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER SDS coolaber Cat#CS9701 Triton X-100 Solution (10%, Sterile) Beyotime CatST797 Tween 20 Beyotime Cat#ST825 DiR membrane dye probe Meilunbio Cat#MB12482 Dio membrane dye probe Meilunbio Cat#MB4239-1 Western and IP cell lysates Beyotime Cat#P0013 Protease inhibitor Mixture (generic, 100×) Beyotime Cat#P1005 Protease phosphatase inhibitor mixture Beyotime Cat#P1045 RIPA lysate Beyotime Cat#P0013B Loading buffer Solarbio Cat#D8371 PVDF membrane Merck millipore Cat#IPVH0010 Matrigel Corning Cat#354234 Goat serum Beyotime Cat#C0265 DAPI dye solution Beyotime Cat#C1005 Hoechst 33342 Mengbio Cat#MBR007 4% paraformaldehyde tissue fixative Biosharp Cat#BL539A Glycine Solaribio Cat#G8200 APS Solaribio Cat#A1010-500 10%SDS Solution Solaribio Cat#S1010 1M Tris-Hcl Buffer Solaribio Cat#T1020 1.5M Tris-Hcl Buffer Solaribio Cat#T1010 OCT frozen section embedding agent SAKURA Cat#4583 Methylprenylated hyaluronic acid EFL Cat#EFL-HAMA-150K Luciferase substrate Dowobio Cat#DW3113 Microfil Flow tech Inc Cat#MV-117 DNase I Roche Cat#10104159001 Recombinant Human Flt3-Ligand PeproTech Cat#300-19 Recombinant Human GM-CSF PeproTech Cat#300-03 Recombinant Human M-CSF PeproTech Cat#300-25 Recombinant Human TGF-β1 PeproTech Cat#100-21 SB265610 MACKLIN Cat#S921626 ZINC00640089 MedChemExpress Cat#667880-11-7 GM6001 aladdin Cat#G274767 Recombinant human MMP2 protein Solarbio Cat#P02344 ANG 193109 MedChemExpress Cat#HY-U00449 LG10268 MedChemExpress Cat#HY-15340 Corn oil MedChemExpress Cat#HY-Y1888 Critical commercial assays Mouse IL-4 ELISA Kit Beyotime Cat#PI612 Mouse IL-10 ELISA Kit Beyotime Cat#PI522 Mouse IFN-γ ELISA Kit Beyotime Cat#PI508 Modified Sirius red staining kit Solarbio Cat#G1472 Modified Masson staining kit Solarbio Cat#G1346 BCA protein concentration determination kit Beyotime Cat#P0012 TSA Fluorescence Triple Staining Kit ABclonal Cat#RK05903 Cell Counting Kit-8 Beyotime Cat#C0039 Annexin V-APC / PI Apoptosis Detection Kit Elabscience Cat#E-CK-A217 10× Genomics Chromium Genetic Analyzer 10× Genomics RRID:SCR_019326 (Continued on next page) e2 Cell Reports Medicine 7, 102698, April 21, 2026 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Biotinylated Human FAP Protein, His,AvitagTM (MALS verified) ACROBiosystems Cat#FAP-H82Q6 Spherotech Streptavidin Polystyrene Particles, 0.5%w/v, 5.0–5.9 μm Spherotech Cat#SVP-50-5 Alexa Fluor 488 NHS ESTER Invitrogen Cat#A20000 Agilent Bioanalyzer Agilent RRID:SCR_018043 Biological samples Human kidney tissue slice Department of Pathology at the Eighth Medical Center of Chinese PLA General Hospital N/A Deposited data scRNA-seq data of mice This paper GEO: GSE252943 (https://www. ncbi.nlm.nih.gov/geo/query/ acc.cgi?acc=GSE252943) scRNA-seq data of human Abedini et al.53 GEO: GSE211785 (https://www. ncbi.nlm.nih.gov/geo/query/ acc.cgi?acc=GSE211785) Bulk-RNA sequencing data This paper GEO: GSE282078 (https://www. ncbi.nlm.nih.gov/geo/query/ acc.cgi?acc=GSE282078) Experimental models: Cell lines Human Umbilical Venous Endothelial Cells (HUVEC) ATCC Cat#bio-81814 Primary human renal fibroblasts icellbioscience Cat#HUM-iCell-u013 THP-1 ATCC Cat#bio-73174 Mouse bone marrow-derived macrophages (BMDMs) This paper N/A hPSC-M Jun Shen lab (State key laboratory of Experimental Hematology)61 N/A Experimental models: Organisms/strains Mouse: balb/C Shanghai Model Organisms Center Cat#SM-003 Mouse: Rosa-LSL-RSR-tdTomato-DTR Shanghai Model Organisms Center N/A Mouse: Cxcr2-DreERT2 This paper N/A Mouse: Cdh5-CreERT2 Shanghai Model Organisms Center Cat#NM-KI-200173 Mouse: Cdh5-CreERT2; Cxcr2-DreERT2; Rosa-LSL-RSR-tdTomato-DTR This paper N/A Software and algorithms ImageJ https://imagej-nihgov.gate2.inist.fr/ij/ N/A FlowJo https://www.flowjo.com 10.8.1 Prism Graphpad https://www.graphpad.com/features Version10 Cell Ranger https://10xgenomics.com/ 7.0.1 R https://www.r-project.org/ 4.1.2 Seurat https://satijalab.org/seurat 5.0.1 Harmony https://github.com/immunogenomics/ harmony 0.1.1 msigdbr https://github.com/igordot/msigdbr 7.5.1 CellChat https://github.com/sqjin/CellChat 1.6.1 SCENIC https://github.com/aertslab/SCENIC 1.3.1 devtools https://CRAN.R-project.org/ package=devtools 2.4.5 ComplexHeatmap N/A 2.9.3 (Continued on next page) Cell Reports Medicine 7, 102698, April 21, 2026 e3 Article ll OPEN ACCESS
Blocking Assay:Article Title: METTL3 regulates α-KG-dependent mitophagy and apoptosis in nucleus pulposus cells through the MALAT1/miR-23c/IDH1 axis.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-ACAN Affinity Cat#DF7561; RRID: AB_2841055 Rabbit anti-COLL II Proteintech Cat#28459-1-AP; RRID: AB_2881147 Rabbit anti-MMP13 Proteintech Cat#18165-1-AP; RRID: AB_2144858 Rabbit anti-ADAMTS5 Affinity Cat#DF13268; RRID: AB_2846287 Rabbit anti-GAPDH Proteintech Cat#60004-1-Ig; RRID: AB_2107436 Rabbit anti-IL-6 Abcam Cat#ab233706; RRID: AB_2889391 Rabbit anti-TNF-α Abcam Cat#ab183218; RRID: AB_2889388 Rabbit anti-Cleaved PARP-1 ThermoFisher Cat#44-698G; RRID: AB_2533726 Rabbit anti-Cleaved Caspase-3 Cell Signaling Technology Cat#9661; RRID: AB_2341188 Rabbit anti-BAX Proteintech Cat#50599-2; RRID: AB_2061561 Rabbit anti-BCL2 Affinity Cat#AF6139; RRID: AB_2835021 Rabbit anti-CAT Abclonal Cat#A11777; RRID: AB_2758747 Rabbit anti-SOD1 Abcam Cat#ab13498; RRID: AB_300402 Rabbit anti-p62 Proteintech Cat#18420-1-AP; RRID: AB_10694431 Rabbit anti-LC3B-II Cell Signaling Technology Cat#2775S; RRID: AB_915950 Rabbit anti-PINK1 Abcam Cat#ab216144; RRID: AB_2927726 Rabbit anti-Parkin Abcam Cat#ab315376; RRID: AB_3668762 Rabbit anti-p-Parkin Thermo Fisher Cat#PA5-114616; RRID: AB_2899252 Rabbit anti-Ubiquitin-Ser65 Boston Biochem Cat#A-110; RRID: AB_2636932 Rabbit anti-IDH1 Abcam Cat#ab256557; RRID: AB_3099653 Rabbit anti-IDH2 Abcam Cat#ab131263; RRID: AB_11156098 Rabbit anti-METTL3 Cell Signaling Technology Cat#86132; RRID: AB_2800072 Biological samples Human nucleus pulposus tissue This paper N/A HEK 293T cells ATCC Cat# CRL-11268 Chemicals, peptides, and recombinant proteins Type II collagenase Invitrogen Cat#17101015 DMEM/F12 medium Gibco Cat#11320033 Fetal bovine serum Gibco Cat#10099141C Penicillin/streptomycin Gibco Cat#15140122 TRIzol reagent Invitrogen Cat15596018CN RIPA Lysis Buffer Beyotime Cat#P0013B Phenylmethanesulfonyl fluoride Beyotime Cat#ST505 DAPI Solarbio Cat#C0065 Actinomycin D MedChem Cat#HY-17559 QuickBlock TM Blocking Buffer Beyotime Cat#P0220 Lipofectamine TM 2000 Invitrogen Cat#11668019 Critical commercial assays α-KG Assay Kit GraceBiotechnology Cat#G0861W Cell Counting Kit-8 Beyotime Cat#C0037 Senescence β-Galactosidase Staining Kit Beyotime Cat#C0602 EdU Cell Proliferation Kit with Alexa Fluor 594 Beyotime Cat#C0078S BCA Protein Assay Kit Beyotime Cat#P0011 (Continued on next page) 20 Cell Reports 45, 116793, January 27, 2026 .. Small pieces of NP tissue that were intraoperatively collected were subjected to 8 h of digestion with 0.2% concentration of type II collagenase (Invitrogen, USA) at 37 ◦ C, followed by 5 min of centrifugation at 1200 rpm.
Inverted Microscopy:Article Title: NSD2 drives triple-negative breast cancer progression via ATAD3A-dependent senescence.
Article Snippet: Triple-negative breast cancer (TNBC) represents the most aggressive subtype of breast cancer, with limited therapeutic options and dismal prognosis.. Histone methyltransferase NSD2 and its catalytic product H3K36me2 are established drivers of TNBC metastasis; however, the downstream effectors linking epigenetic regulation to cellular senescence remain largely unknown.. This study utilized transcriptomic profiling, ChIP-qPCR, and in vivo xenograft models to define the function and molecular mechanism of the NSD2-ATAD3A axis.
Counting:Article Title: NSD2 drives triple-negative breast cancer progression via ATAD3A-dependent senescence.
Article Snippet: Triple-negative breast cancer (TNBC) represents the most aggressive subtype of breast cancer, with limited therapeutic options and dismal prognosis.. Histone methyltransferase NSD2 and its catalytic product H3K36me2 are established drivers of TNBC metastasis; however, the downstream effectors linking epigenetic regulation to cellular senescence remain largely unknown.. This study utilized transcriptomic profiling, ChIP-qPCR, and in vivo xenograft models to define the function and molecular mechanism of the NSD2-ATAD3A axis.
Cell Counting:
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